Protocol for the isolation of the mouse sympathetic splanchnic-celiac-superior mesenteric ganglion complex

Pedro Trevizan-Baú; Mitchell Ty Ringuet; Martin J. Stebbing; Robin M. McAllen; John B. Furness; Scott N. Mueller

STAR Protocols (Jun 2024)

Protocol for the isolation of the mouse sympathetic splanchnic-celiac-superior mesenteric ganglion complex

Pedro Trevizan-Baú,
Mitchell Ty Ringuet,
Martin J. Stebbing,
Robin M. McAllen,
John B. Furness,
Scott N. Mueller

Affiliations

Pedro Trevizan-Baú: The Florey Institute, The University of Melbourne, Melbourne, VIC, Australia; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, VIC, Australia; Department of Physiological Sciences, University of Florida, Gainesville, FL, USA; Corresponding author
Mitchell Ty Ringuet: The Florey Institute, The University of Melbourne, Melbourne, VIC, Australia; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, VIC, Australia
Martin J. Stebbing: The Florey Institute, The University of Melbourne, Melbourne, VIC, Australia
Robin M. McAllen: The Florey Institute, The University of Melbourne, Melbourne, VIC, Australia
John B. Furness: The Florey Institute, The University of Melbourne, Melbourne, VIC, Australia; Department of Anatomy and Physiology, University of Melbourne, Parkville, VIC, Australia
Scott N. Mueller: Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, VIC, Australia; Corresponding author

Journal volume & issue: Vol. 5, no. 2
p. 103036

Abstract

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Summary: Neurons that originate from pre-vertebral sympathetic ganglia, the splanchnic-celiac-superior mesenteric ganglion complex (SCSMG) in mouse, have important roles in control of organs of the upper abdomen. Here, we present a protocol for the isolation of the mouse sympathetic SCSMG. We describe steps for surgical incision, ganglia isolation, ganglia fine dissection, and whole-mount SCSMG after clearing-enhanced 3D (Ce3D) clearing method and immunohistochemistry. Given the importance of mice in studies of that control, this protocol aims to assist biomedical researchers in the dissection of the mouse SCSMG. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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