<i>Lactobacillus reuteri</i> DSM 17938 Protects against Gastric Damage Induced by Ethanol Administration in Mice: Role of TRPV1/Substance P Axis
Ana P. Oliveira,
Luan K. M. Souza,
Thiago S. L. Araújo,
Simone de Araújo,
Kerolayne M. Nogueira,
Francisca Beatriz M. Sousa,
Renan O. Silva,
Dvison M. Pacífico,
Conceição S. Martins,
Gerly Anne de C. Brito,
Marcellus H.L.P. Souza,
Jand Venes R. Medeiros
Affiliations
Ana P. Oliveira
Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (Lafidg), Federal University of Piauí, Av. São Sebastião, nº 2819, CEP 64202-02, Parnaíba, PI, Brazil
Luan K. M. Souza
Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (Lafidg), Federal University of Piauí, Av. São Sebastião, nº 2819, CEP 64202-02, Parnaíba, PI, Brazil
Thiago S. L. Araújo
Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (Lafidg), Federal University of Piauí, Av. São Sebastião, nº 2819, CEP 64202-02, Parnaíba, PI, Brazil
Simone de Araújo
Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (Lafidg), Federal University of Piauí, Av. São Sebastião, nº 2819, CEP 64202-02, Parnaíba, PI, Brazil
Kerolayne M. Nogueira
Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (Lafidg), Federal University of Piauí, Av. São Sebastião, nº 2819, CEP 64202-02, Parnaíba, PI, Brazil
Francisca Beatriz M. Sousa
Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (Lafidg), Federal University of Piauí, Av. São Sebastião, nº 2819, CEP 64202-02, Parnaíba, PI, Brazil
Renan O. Silva
Department of Physiology and Pharmacology, Federal University of Ceará, CEP 60430-270, Fortaleza, Ceará, Brazil
Dvison M. Pacífico
Postgraduate Program in Morphofunctional Sciences, Department of Morphology, Faculty of Medicine, Federal University Ceará, CEP 60430-170, Fortaleza-CE, Brazil
Conceição S. Martins
Postgraduate Program in Morphofunctional Sciences, Department of Morphology, Faculty of Medicine, Federal University Ceará, CEP 60430-170, Fortaleza-CE, Brazil
Gerly Anne de C. Brito
Postgraduate Program in Morphofunctional Sciences, Department of Morphology, Faculty of Medicine, Federal University Ceará, CEP 60430-170, Fortaleza-CE, Brazil
Marcellus H.L.P. Souza
Department of Physiology and Pharmacology, Federal University of Ceará, CEP 60430-270, Fortaleza, Ceará, Brazil
Jand Venes R. Medeiros
Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (Lafidg), Federal University of Piauí, Av. São Sebastião, nº 2819, CEP 64202-02, Parnaíba, PI, Brazil
This study aimed to evaluate the effect of Lactobacillus reuteri DSM 17938 (DSM) on ethanol-induced gastric injury, and if its possible mechanism of action is related to inhibiting the transient receptor potential vanilloid type 1 (TRPV1). We evaluated the effect of supplementing 108 CFU•g body wt−1•day−1 of DSM on ethanol-induced gastric injury. DSM significantly reduced the ulcer area (1.940 ± 1.121 mm2) with 3 days of pretreatment. The effects of DSM supplementation were reversed by Resiniferatoxin (RTX), TRPV1 agonist (3 nmol/kg p.o.). Substance P (SP) (1 μmol/L per 20 g) plus 50% ethanol resulted in hemorrhagic lesions, and DSM supplementation did not reverse the lesion area induced by administering SP. TRPV1 staining intensity was lower, SP, malondialdehyde (MDA) and nitrite levels were reduced, and restored normal levels of antioxidant parameters (glutathione and superoxide dismutase) in the gastric mucosa in mice treated with DSM. In conclusion, DSM exhibited gastroprotective activity through decreased expression of TRPV1 receptor and decreasing SP levels, with a consequent reduction of oxidative stress.
