Frontiers in Cellular and Infection Microbiology (Aug 2017)
Use of Digital Droplet PCR to Detect Mycobacterium tuberculosis DNA in Whole Blood-Derived DNA Samples from Patients with Pulmonary and Extrapulmonary Tuberculosis
- Jiaru Yang,
- Jiaru Yang,
- Jiaru Yang,
- Jiaru Yang,
- Jiaru Yang,
- Xinlin Han,
- Xinlin Han,
- Xinlin Han,
- Xinlin Han,
- Xinlin Han,
- Aihua Liu,
- Aihua Liu,
- Aihua Liu,
- Aihua Liu,
- Aihua Liu,
- Xiyuan Bai,
- Cuiping Xu,
- Cuiping Xu,
- Cuiping Xu,
- Cuiping Xu,
- Cuiping Xu,
- Fukai Bao,
- Fukai Bao,
- Fukai Bao,
- Fukai Bao,
- Fukai Bao,
- Shi Feng,
- Lvyan Tao,
- Lvyan Tao,
- Lvyan Tao,
- Lvyan Tao,
- Lvyan Tao,
- Mingbiao Ma,
- Mingbiao Ma,
- Mingbiao Ma,
- Mingbiao Ma,
- Mingbiao Ma,
- Yun Peng,
- Yun Peng,
- Yun Peng,
- Yun Peng,
- Yun Peng
Affiliations
- Jiaru Yang
- Yunnan Key Laboratory for Tropical Infectious DiseasesKunming, China
- Jiaru Yang
- Yunnan Collaborative Innovation Center for Public Health and Disease ControlKunming, China
- Jiaru Yang
- Institute for Tropical Medicine, Kunming Medical UniversityKunming, China
- Jiaru Yang
- Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Kunming Medical UniversityKunming, China
- Jiaru Yang
- Yunnan Province Base for International Scientific and Technological Cooperation in Tropical DiseasesKunming, China
- Xinlin Han
- Yunnan Key Laboratory for Tropical Infectious DiseasesKunming, China
- Xinlin Han
- Yunnan Collaborative Innovation Center for Public Health and Disease ControlKunming, China
- Xinlin Han
- Institute for Tropical Medicine, Kunming Medical UniversityKunming, China
- Xinlin Han
- Yunnan Province Base for International Scientific and Technological Cooperation in Tropical DiseasesKunming, China
- Xinlin Han
- Department of Microbiology and Immunology, School of Basic Medical Science, Kunming Medical UniversityKunming, China
- Aihua Liu
- Yunnan Key Laboratory for Tropical Infectious DiseasesKunming, China
- Aihua Liu
- Yunnan Collaborative Innovation Center for Public Health and Disease ControlKunming, China
- Aihua Liu
- Institute for Tropical Medicine, Kunming Medical UniversityKunming, China
- Aihua Liu
- Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Kunming Medical UniversityKunming, China
- Aihua Liu
- Yunnan Province Base for International Scientific and Technological Cooperation in Tropical DiseasesKunming, China
- Xiyuan Bai
- Departments of Medicine and Academic Affairs, National Jewish HealthDenver, CO, United States
- Cuiping Xu
- Yunnan Key Laboratory for Tropical Infectious DiseasesKunming, China
- Cuiping Xu
- Yunnan Collaborative Innovation Center for Public Health and Disease ControlKunming, China
- Cuiping Xu
- Institute for Tropical Medicine, Kunming Medical UniversityKunming, China
- Cuiping Xu
- Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Kunming Medical UniversityKunming, China
- Cuiping Xu
- Yunnan Province Base for International Scientific and Technological Cooperation in Tropical DiseasesKunming, China
- Fukai Bao
- Yunnan Key Laboratory for Tropical Infectious DiseasesKunming, China
- Fukai Bao
- Yunnan Collaborative Innovation Center for Public Health and Disease ControlKunming, China
- Fukai Bao
- Institute for Tropical Medicine, Kunming Medical UniversityKunming, China
- Fukai Bao
- Yunnan Province Base for International Scientific and Technological Cooperation in Tropical DiseasesKunming, China
- Fukai Bao
- Department of Microbiology and Immunology, School of Basic Medical Science, Kunming Medical UniversityKunming, China
- Shi Feng
- Department of Microbiology and Immunology, School of Basic Medical Science, Kunming Medical UniversityKunming, China
- Lvyan Tao
- Yunnan Key Laboratory for Tropical Infectious DiseasesKunming, China
- Lvyan Tao
- Yunnan Collaborative Innovation Center for Public Health and Disease ControlKunming, China
- Lvyan Tao
- Institute for Tropical Medicine, Kunming Medical UniversityKunming, China
- Lvyan Tao
- Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Kunming Medical UniversityKunming, China
- Lvyan Tao
- Yunnan Province Base for International Scientific and Technological Cooperation in Tropical DiseasesKunming, China
- Mingbiao Ma
- Yunnan Key Laboratory for Tropical Infectious DiseasesKunming, China
- Mingbiao Ma
- Yunnan Collaborative Innovation Center for Public Health and Disease ControlKunming, China
- Mingbiao Ma
- Institute for Tropical Medicine, Kunming Medical UniversityKunming, China
- Mingbiao Ma
- Yunnan Province Base for International Scientific and Technological Cooperation in Tropical DiseasesKunming, China
- Mingbiao Ma
- Department of Microbiology and Immunology, School of Basic Medical Science, Kunming Medical UniversityKunming, China
- Yun Peng
- Yunnan Key Laboratory for Tropical Infectious DiseasesKunming, China
- Yun Peng
- Yunnan Collaborative Innovation Center for Public Health and Disease ControlKunming, China
- Yun Peng
- Institute for Tropical Medicine, Kunming Medical UniversityKunming, China
- Yun Peng
- Yunnan Province Base for International Scientific and Technological Cooperation in Tropical DiseasesKunming, China
- Yun Peng
- Department of Microbiology and Immunology, School of Basic Medical Science, Kunming Medical UniversityKunming, China
- DOI
- https://doi.org/10.3389/fcimb.2017.00369
- Journal volume & issue
-
Vol. 7
Abstract
Tuberculosis (TB) is a chronic infectious disease that has been threatening public health for many centuries. The clinical diagnostic procedure for TB is time-consuming and laborious. In the last 20 years, real-time fluorescence-based quantitative PCR (real-time PCR) has become a better alternative for TB diagnosis in clinics due to its sensitivity and specificity. Recently, digital droplet PCR (ddPCR) has been developed, and it might be an ideal alternative to conventional real-time PCR for microorganism detection. In this study, we aimed to assess the capacity of ddPCR and real-time PCR for detecting low levels of circulating Mycobacterium tuberculosis (MTB) DNA. The study involved testing whole blood samples for an MTB DNA target (known as IS6110). Blood samples were obtained from 28 patients with pulmonary TB, 28 patients with extrapulmonary TB, and 28 healthy individuals. The results show that ddPCR could be used to measure low levels of MTB DNA, and it has the potential to be used to diagnose pulmonary and extrapulmonary TB based on clinical samples.
Keywords