Frontiers in Chemistry (Nov 2021)

Single-Conidium Encapsulation in Oil-in-Water Pickering Emulsions at High Encapsulation Yield

  • Liliya Kotliarevski,
  • Liliya Kotliarevski,
  • Karthik Ananth Mani,
  • Karthik Ananth Mani,
  • Reut Amar Feldbaum,
  • Noga Yaakov,
  • Eduard Belausov,
  • Einat Zelinger,
  • Dana Ment,
  • Guy Mechrez

DOI
https://doi.org/10.3389/fchem.2021.726874
Journal volume & issue
Vol. 9

Abstract

Read online

This study presents an individual encapsulation of fungal conidia in an oil-in-water Pickering emulsion at a single-conidium encapsulation yield of 44%. The single-conidium encapsulation yield was characterized by analysis of confocal microscopy micrographs. Mineral oil-in-water emulsions stabilized by amine-functionalized titania dioxide (TiO2-NH2 or titania-NH2) particles were prepared. The structure and the stability of the emulsions were investigated at different compositions by confocal microscopy and a LUMiSizer® respectively. The most stable emulsions with a droplet size suitable for single-conidium encapsulation were further studied for their individual encapsulation capabilities. The yields of individual encapsulation in the emulsions; i.e., the number of conidia that were individually encapsulated out of the total number of conidia, were characterized by confocal microscopy assay. This rapid, easy to use approach to single-conidium encapsulation, which generates a significantly high yield with eco-friendly titania-based emulsions, only requires commonly used emulsification and agitation methods.

Keywords