Single-Conidium Encapsulation in Oil-in-Water Pickering Emulsions at High Encapsulation Yield

Liliya Kotliarevski; Liliya Kotliarevski; Karthik Ananth Mani; Karthik Ananth Mani; Reut Amar Feldbaum; Noga Yaakov; Eduard Belausov; Einat Zelinger; Dana Ment; Guy Mechrez

doi:10.3389/fchem.2021.726874

Frontiers in Chemistry (Nov 2021)

Single-Conidium Encapsulation in Oil-in-Water Pickering Emulsions at High Encapsulation Yield

Liliya Kotliarevski,
Liliya Kotliarevski,
Karthik Ananth Mani,
Karthik Ananth Mani,
Reut Amar Feldbaum,
Noga Yaakov,
Eduard Belausov,
Einat Zelinger,
Dana Ment,
Guy Mechrez

Affiliations

Liliya Kotliarevski: Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Institute, Rishon Lezion, Israel
Liliya Kotliarevski: Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Karthik Ananth Mani: Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Institute, Rishon Lezion, Israel
Karthik Ananth Mani: Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Reut Amar Feldbaum: Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Institute, Rishon Lezion, Israel
Noga Yaakov: Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Institute, Rishon Lezion, Israel
Eduard Belausov: Department of Ornamental Plants and Agricultural Biotechnology, Institute of Plant Science, Agricultural Research Organization (ARO), Volcani Institute, Rishon Lezion, Israel
Einat Zelinger: Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Dana Ment: Department of Plant Pathology and Weed Research, Institute of Plant Protection, Agricultural Research Organization (ARO), Volcani Institute, Rishon Lezion, Israel
Guy Mechrez: Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Institute, Rishon Lezion, Israel

DOI: https://doi.org/10.3389/fchem.2021.726874
Journal volume & issue: Vol. 9

Abstract

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This study presents an individual encapsulation of fungal conidia in an oil-in-water Pickering emulsion at a single-conidium encapsulation yield of 44%. The single-conidium encapsulation yield was characterized by analysis of confocal microscopy micrographs. Mineral oil-in-water emulsions stabilized by amine-functionalized titania dioxide (TiO2-NH2 or titania-NH2) particles were prepared. The structure and the stability of the emulsions were investigated at different compositions by confocal microscopy and a LUMiSizer® respectively. The most stable emulsions with a droplet size suitable for single-conidium encapsulation were further studied for their individual encapsulation capabilities. The yields of individual encapsulation in the emulsions; i.e., the number of conidia that were individually encapsulated out of the total number of conidia, were characterized by confocal microscopy assay. This rapid, easy to use approach to single-conidium encapsulation, which generates a significantly high yield with eco-friendly titania-based emulsions, only requires commonly used emulsification and agitation methods.

Published in Frontiers in Chemistry

ISSN: 2296-2646 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Chemistry
Website: http://journal.frontiersin.org/journal/chemistry

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