iScience (Apr 2025)
Dynamic changes of molecular pattern and cellular subpopulation in puncture-induced tendon injury model
- Zizhan Huang,
- Ziyang Li,
- Dengfeng Ruan,
- Yiwen Xu,
- Honglu Cai,
- Hengzhi Liu,
- Haocheng Jin,
- Peiwen He,
- Yang Fei,
- Jiayun Huang,
- Canlong Wang,
- Xiao Chen,
- Jia Jiang,
- Weiliang Shen
Affiliations
- Zizhan Huang
- Department of Sports Medicine & Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, P.R. China; Institute of Sports Medicine, Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Zhejiang Key Laboratory of Motor System Disease Precision Research and Therapy, Hangzhou City, Zhejiang Province, P.R. China; Clinical Research Center of Motor System Disease of Hangzhou City, Hangzhou City, Zhejiang Province, P.R. China
- Ziyang Li
- Department of Sports Medicine & Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, P.R. China; Institute of Sports Medicine, Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Zhejiang Key Laboratory of Motor System Disease Precision Research and Therapy, Hangzhou City, Zhejiang Province, P.R. China; Clinical Research Center of Motor System Disease of Hangzhou City, Hangzhou City, Zhejiang Province, P.R. China
- Dengfeng Ruan
- Department of Sports Medicine & Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, P.R. China; Institute of Sports Medicine, Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Zhejiang Key Laboratory of Motor System Disease Precision Research and Therapy, Hangzhou City, Zhejiang Province, P.R. China; Clinical Research Center of Motor System Disease of Hangzhou City, Hangzhou City, Zhejiang Province, P.R. China; China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China
- Yiwen Xu
- Department of Sports Medicine & Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, P.R. China; Institute of Sports Medicine, Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Zhejiang Key Laboratory of Motor System Disease Precision Research and Therapy, Hangzhou City, Zhejiang Province, P.R. China; Clinical Research Center of Motor System Disease of Hangzhou City, Hangzhou City, Zhejiang Province, P.R. China
- Honglu Cai
- Department of Sports Medicine & Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, P.R. China; Institute of Sports Medicine, Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Zhejiang Key Laboratory of Motor System Disease Precision Research and Therapy, Hangzhou City, Zhejiang Province, P.R. China; Clinical Research Center of Motor System Disease of Hangzhou City, Hangzhou City, Zhejiang Province, P.R. China
- Hengzhi Liu
- Department of Sports Medicine & Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, P.R. China; Institute of Sports Medicine, Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Zhejiang Key Laboratory of Motor System Disease Precision Research and Therapy, Hangzhou City, Zhejiang Province, P.R. China; Clinical Research Center of Motor System Disease of Hangzhou City, Hangzhou City, Zhejiang Province, P.R. China
- Haocheng Jin
- Department of Orthopedics, National Center for Orthopedics, Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China
- Peiwen He
- Department of Sports Medicine & Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, P.R. China; Institute of Sports Medicine, Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Zhejiang Key Laboratory of Motor System Disease Precision Research and Therapy, Hangzhou City, Zhejiang Province, P.R. China; Clinical Research Center of Motor System Disease of Hangzhou City, Hangzhou City, Zhejiang Province, P.R. China
- Yang Fei
- Department of Sports Medicine & Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, P.R. China; Institute of Sports Medicine, Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Zhejiang Key Laboratory of Motor System Disease Precision Research and Therapy, Hangzhou City, Zhejiang Province, P.R. China; Clinical Research Center of Motor System Disease of Hangzhou City, Hangzhou City, Zhejiang Province, P.R. China
- Jiayun Huang
- Department of Sports Medicine & Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, P.R. China; Institute of Sports Medicine, Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Zhejiang Key Laboratory of Motor System Disease Precision Research and Therapy, Hangzhou City, Zhejiang Province, P.R. China; Clinical Research Center of Motor System Disease of Hangzhou City, Hangzhou City, Zhejiang Province, P.R. China
- Canlong Wang
- Department of Sports Medicine & Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, P.R. China; Institute of Sports Medicine, Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Zhejiang Key Laboratory of Motor System Disease Precision Research and Therapy, Hangzhou City, Zhejiang Province, P.R. China; Clinical Research Center of Motor System Disease of Hangzhou City, Hangzhou City, Zhejiang Province, P.R. China
- Xiao Chen
- Department of Sports Medicine & Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, P.R. China; Zhejiang Key Laboratory of Motor System Disease Precision Research and Therapy, Hangzhou City, Zhejiang Province, P.R. China; Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, P.R. China; Liangzhu Laboratory, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou City, P.R. China; State Key Laboratory of Transvascular Implantation Devices, Hangzhou City, Zhejiang Province, P.R. China; China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China; Corresponding author
- Jia Jiang
- Department of Orthopedics, National Center for Orthopedics, Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China; Corresponding author
- Weiliang Shen
- Department of Sports Medicine & Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, P.R. China; Institute of Sports Medicine, Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, P.R. China; Zhejiang Key Laboratory of Motor System Disease Precision Research and Therapy, Hangzhou City, Zhejiang Province, P.R. China; Clinical Research Center of Motor System Disease of Hangzhou City, Hangzhou City, Zhejiang Province, P.R. China; Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, P.R. China; Liangzhu Laboratory, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou City, P.R. China; State Key Laboratory of Transvascular Implantation Devices, Hangzhou City, Zhejiang Province, P.R. China; China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China; Corresponding author
- Journal volume & issue
-
Vol. 28,
no. 4
p. 112034
Abstract
Summary: Tendon degeneration and injury often result in significant pain and functional impairment. Typically, tendon healing occurs through a scar-mediated response and may progress to chronic tendinopathy without timely intervention. However, the molecular mechanisms underlying early tendon repair remain poorly understood. Further investigation is also impeded by the limited availability of early tendon injury samples in clinical settings. In this study, we established a puncture-induced tendon injury model to investigate the molecular patterns and cellular subpopulations involved in early tendon injury across multiple time points. RNA sequencing identified seven gene sets with distinct expression profiles during the early stages of tendon injury. Single-cell RNA sequencing further revealed eight myeloid cell types and seven mesenchymal cell types participating in the tendon repair process. Together, these findings illuminate the molecular and cellular dynamics coordinating early tendon repair, providing insights that could inform future clinical treatments for tendinopathy and tendon injury.
