精准医学杂志 (Aug 2023)

EFFECTS OF INDOLE-2,3-DIONE ON THE PROLIFERATION AND MIGRATION OF SH-SY5Y CELLS AND UNDERLYING MECHANISMS

  • QIU Biru, HOU Lin, ZHANG Li, NIU Tingting, SONG Junying

DOI
https://doi.org/10.13362/jj.pmed.202304016
Journal volume & issue
Vol. 38, no. 4
pp. 360 – 364

Abstract

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Objective To investigate the effects of indole-2,3-dione (ISA) on the proliferation and migration of SH-SY5Y cells and the underlying mechanisms. Methods CCK-8 assay was used to assess the effect of ISA on the viability of SH-SY5Y cells, and half maximal inhibitory concentration (IC50) was calculated and used as the reference value of concentration setting in subsequent experiments. On the basis of IC50, SH-SY5Y cells were added with ISA at the final concentrations of 0, 50, 100, and 200 μmol/L (groups A, B, C, D, respectively). The effects of ISA on the proliferation, migration, apoptosis, and cell cycle of SH-SY5Y cells were determined by plate cloning, scratch assay, cell apoptosis rate detection, and PI staining flow cytometry analysis. Western blot assay was used to determine the relative expression of proteins such as Bax and Bcl2 in SH-SY5Y cells. Results The CCK-8 assay showed that the activity of SH-SY5Y cells decreased significantly with the increase in ISA concentration (F=1 632.62,P<0.05). Plate cloning showed that the colony formation ability of the cells was inhibited with the increase in ISA concentration (F=1 038.21,P<0.05). Scratch test showed that the migration ability of cells decreased significantly with the increase in ISA concentration (F=147.86,P<0.05). Flow cytometry showed that with the increase in ISA concentration, the apoptosis rate of cells increased significantly (F=557.71,P<0.05). PI staining showed that with the increase in ISA concentration, the proportion of cells in the G1 phase increased significantly (F=632.38,P<0.05). Western blot showed that with the increase in ISA concentration, the expression of p-JAK2, p-STAT3, and Bcl2 decreased significantly (F=286.56-541.13,P<0.05), while the expression of Bax increased significantly (F=464.50,P<0.05). Conclusion ISA can effectively inhibit the proliferation and migration of neuroblastoma SH-SY5Y cells, which may be achieved by regulating the JAK2/STAT3 pathway.

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