Effects of Mangifera indica leaves improves blood lipids profile and biochemical indices in high-fat diet-induced hyperlipidaemia rats

Abstract

Dyslipidemia a chronic, metabolic syndrome characterized by elevated lipid profiles together with lipid peroxidation in individuals with atherosclerotic cardiovascular disease (CVD). Functional ingredients such as high phenolic content and potent antioxidant activity obtained from agricultural waste by-products or waste are of great interest. However, the hypolipidemic effects of the waste mango (Mangifera indica L.) leaves (MLE) have not been investigated. Here, the specific lipid-lowering and potential hepatoprotective mechanisms by which the gavage administration of MLE affects lipid metabolism and liver steatosis in rats fed on a high-fat diet (HFD) were evaluated. In rats treated with high level of MLE, a persistent suppressive effect on liver weight and body weight gain was discovered after 28-day intervention. Furthermore, body lipid index and reduced inflammatory reaction and liver function parameters in HFD control rats were markedly ameliorated by supplementation with high doses of MLE. In addition, histological and histomorphometric analyses here demonstrated that fat accumulation changed in HFD supplemented hyperlipidaemia rats, but normalized in MLE treatment groups. Further real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) and western blot analyses were carried out to determine the mRNA and protein abundance of PPARα receptors and CYP7A1 in liver tissues of rats. These results indicate that MLE supplement have the promising lipid lowering effects in HFD-induced hyperlipidaemia rats based on positive ameliorations in the serum lipid profile and liver function parameters.