Brazilian Archives of Biology and Technology (Jul 2020)

Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter Study

  • Silvana Spindola de Miranda,
  • Isabela Neves de Almeida,
  • Maria de Fátima Filardi Oliveira Mansur,
  • Lida Jouca de Assis Figueredo,
  • Wânia da Silva Carvalho,
  • João Paulo Amaral Hadaad,
  • Jaciara de Lourdes do Carmo Guimarães Diniz,
  • Andrea von Groll,
  • Pedro Almeida da Silva,
  • Maria Luiza Lopes,
  • Marcelo Cordeiro dos Santos,
  • Alexandra Brito,
  • Fernanda Carvalho de Queiroz Mello,
  • Thiago da Silva Santos Malaquias,
  • Julio Croda,
  • Juliana Maira Watanabe Pinhata,
  • Rosângela Siqueira de Oliveira,
  • Erica Chimara,
  • Maria Lúcia Rossetti,
  • Maria Laura Halon,
  • Maria Cristina Lourenço,
  • Reginalda Ferreira de Melo Medeiros,
  • Fátima Cristina Onofre Fandinho Montes,
  • Diana Machado,
  • Miguel Viveiros,
  • Afrânio Lineu Kritski

DOI
https://doi.org/10.1590/1678-4324-2020190179
Journal volume & issue
Vol. 63

Abstract

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Abstract (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.

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