大肠杆菌细胞膜固定相萃取辣木籽抗菌肽Extraction of Moringa oleifera seed antimicrobial peptides by E.coli cell membrane stationary phase

Abstract

制备大肠杆菌细胞膜固定相(E.coli cell membrane stationary phase，ECMSP)，研究其对辣木籽抗菌肽的萃取效果。研究超声时间、超声功率对大肠杆菌破壁效果的影响，分析大肠杆菌细胞膜与大孔硅胶的吸附特征及ECMSP对辣木籽抗菌肽的吸附效果，明确萃取前后抑菌活力变化及反相-高效液相色谱（RP-HPLC）差异峰。结果表明：在超声功率500 W、超声时间30 min条件下，大肠杆菌破壁效果较好；大孔硅胶对大肠杆菌细胞膜的饱和吸附值（Csmax）为9.98 μg/mg、吸附常数（K*）为171.02 μg/mL；萃取前后抗菌肽对大肠杆菌和金黄色葡萄球菌的抑制效果差异明显，RP-HPLC分析表明萃取前后出现了3个差异峰。研究结果可为辣木籽抗菌肽的后续开发利用提供理论基础和应用参考。 E. coli cell membrane stationary phase (ECMSP) was prepared, and its extraction effect of Moringa oleifera seed antimicrobial peptides was studied. The effects of ultrasonic time and ultrasonic power on the wall breaking effect of E. coli were studied. The adsorption characteristics of E. coli cell membrane and macroporous silica gel, and the adsorption effect of ECMSP on the Moringa oleifera seed antimicrobial peptides were analyzed. The change of antibacterial activity and the reverse-high performance liquid chromatography (RP-HPLC) difference peaks before and after extraction were clarified. The results showed that when the ultrasonic power was 500 W and the ultrasonic time was 30 min, the wall breaking effect was good. The saturated adsorption value （Csmax）of the macroporous silica gel to the cell membrane of E. coli was 9.98 μg/mg, and the adsorption constant (K*) was 171.02 μg/mL. The inhibitory effects of antimicrobial peptides on Escherichia coli and Staphylococcus aureus were significantly different, and RP-HPLC analysis showed three different peaks before and after extraction. The research could provide theoretical basis and application references for the subsequent development and utilization of Moringa oleifera seed antimicrobial peptides.

Keywords

• 大肠杆菌；大肠杆菌细胞膜固定相；辣木籽抗菌肽；萃取；抑菌率
• escherichia coli; e.coli cell membrane stationary phase; moringa oleifera seed antimicrobial peptide; extraction; antibacterial rate