Alternation of gene expression in brain-derived exosomes after cerebral ischemic preconditioning in mice
He Li,
Xiaoxi Zhang,
Hongye Xu,
Hanchen Liu,
Yongxin Zhang,
Lei Zhang,
Yu Zhou,
Yongwei Zhang,
Jianmin Liu,
Mei Jing,
Ping Zhang,
Pengfei Yang
Affiliations
He Li
Emergency Department, Naval Medical Center of PLA, Naval Medical University, Shanghai, China; Neurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China
Xiaoxi Zhang
Neurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China
Hongye Xu
Neurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China
Hanchen Liu
Neurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China
Yongxin Zhang
Neurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China
Lei Zhang
Neurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China
Yu Zhou
Neurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China
Yongwei Zhang
Neurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China
Jianmin Liu
Neurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China
Mei Jing
Emergency Department, Naval Medical Center of PLA, Naval Medical University, Shanghai, China; Corresponding author. Emergency Department, Naval Medical Center of PLA, Naval Medical University, Num.338, West Huaihai Road, Changning District, Shanghai, 200052, China.
Ping Zhang
Neurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China; Department of Neurology, Naval Medical Center of PLA, Naval Medical University, Shanghai, China; Corresponding author. Neurovascular Center, Changhai Hospital, Num.168, Changhai Road, Yangpu District, Shanghai, 200433, China.
Pengfei Yang
Neurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China; Corresponding author. Neurovascular Center, Changhai Hospital, Num.168, Changhai Road, Yangpu District, Shanghai, 200433, China.
Aims: Cerebral ischemic preconditioning is a neuroprotective therapy against cerebral ischemia and ischemia-reperfusion injury. This study aims to demonstrate the alternation of gene expression in exosomes from brain tissue of mice after ischemic preconditioning and their potential functions. Methods: Ten mice were divided into the sham and the cerebral ischemic preconditioning groups. Their brain tissues were harvested, from which the exosomes were extracted. The characteristics and protective effects of exosomes were evaluated. Whole transcriptome sequencing was used to demonstrate the gene expression discrepancy between the exosomes from the two groups of mice brains. Volcano graphs and heatmaps were used to picture the difference in expression quantity of mRNA, lncRNA, and circRNA. Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to demonstrate the functions of differentially expressed RNAs. Results: Exosomes were successfully extracted, and those from the cerebral ischemic preconditioning group had better protective effects on cells that received oxygen-glucose deprivation and restoration injury. A total of 306 mRNAs and 374 lncRNAs were significantly upregulated, and 320 mRNAs and 405 lncRNAs were significantly downregulated in the preconditioning group. No circRNAs were differentially expressed between the two groups. GO and KEGG pathway analysis indicated that the functions of differentially expressed RNAs were related to both neural protective and injurious effects. Conclusion: The brain-derived exosomes may participate in the neuroprotective effect of cerebral ischemic preconditioning. Thorough research is necessary to investigate exosome functions derived from the ischemic preconditioned brain.
