An in-vitro Cytotoxic and Genotoxic Properties of Allmanda Cathartica L. Latex Green NPs on Human Peripheral Blood Mononuclear Cells

Abstract

Green synthesis of silver nanoparticles (NPs) by green route approaches has advantages over conventional methods. In green synthesis, we use eco-friendly plant extracts contain secondary metabolites and bioactive components, proteins that act as both reducing and capping agents, form stable and shape-controlled green silver nanoparticles. The current study deals with the synthesis of silver nanoparticles using the aqueous latex extract of Allmanda cathartica. The green silver nanoparticles are characterized by using different spectroscopic methods like ultra violet-visible spectroscopy (UV-Vis), Fourier transform-infrared spectroscopy (FTIR), transmission electron microscope (TEM), scanning electron microscope (SEM) and X-ray diffraction (XRD). Results indicated that the crystalline natured particles were spherical shaped with an average of 35 nm in size, and that the stability of silver nanoparticles was due to its high negative zeta potential of –27.6 mV. The current study also revealed that green silver nanoparticles had very good genotoxic and cytotoxic activity in peripheral blood mononuclear cells (PBMCs). Leukemia leads to the development of high numbers of white blood cells, which is one of the major types of cancers that affect children. Many of the chemicals used for the treatment produce remarkable side effects. To overcome this problem, we made an attempt to see the efficacy of latex green silver nanoparticle on peripheral blood mononuclear cells and deoxyribonucleic acid fragmentation, which leads to the development of future therapeutic drugs.

Keywords

• genotoxic and cytotoxic studies
• green ag nps
• human pbmcs
• spectral analysis
• sem
• tem
• zeta potential