Establishment of a TNFRSF11B knock-out human induced pluripotent stem cell line (KSCBi002-B-2) via CRISPR/Cas9 system

Jujin Jeong; Dahye Lee; Byeong-Cheol Park; Tae-Hoon Lee; Sang-Wook Park

Stem Cell Research (Sep 2023)

Establishment of a TNFRSF11B knock-out human induced pluripotent stem cell line (KSCBi002-B-2) via CRISPR/Cas9 system

Jujin Jeong,
Dahye Lee,
Byeong-Cheol Park,
Tae-Hoon Lee,
Sang-Wook Park

Affiliations

Jujin Jeong: Department of Oral Biochemistry, School of Dentistry, Chonnam National University, Gwangju 61186, South Korea
Dahye Lee: Department of Oral Biochemistry, School of Dentistry, Chonnam National University, Gwangju 61186, South Korea
Byeong-Cheol Park: Department of Oral Biochemistry, School of Dentistry, Chonnam National University, Gwangju 61186, South Korea
Tae-Hoon Lee: Corresponding authors.; Department of Oral Biochemistry, School of Dentistry, Chonnam National University, Gwangju 61186, South Korea
Sang-Wook Park: Corresponding authors.; Department of Oral Biochemistry, School of Dentistry, Chonnam National University, Gwangju 61186, South Korea

Journal volume & issue: Vol. 71
p. 103167

Abstract

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A TNFRSF11B (TNF Receptor Superfamily Member 11b) gene encodes a soluble decoy receptor, osteoprotegerin (OPG), which has a key role in repressing osteoclast differentiation. In this report, we generated a biallelic knock-out hiPSC line for the TNFRSF11B gene via CRISPR/Cas9. When TNFRSF11B Knock-out hiPSCs were differentiated into mesenchymal progenitor cells (MPCs), the expression level of OPG was significantly decreased compared to normal hiPSC-derived MPCs. This knock-out hiPSCs will provide a chance to study Paget disease of bone 5 (juvenile Paget disease).

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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