An In-Vitro Evaluation of Toxic Metals Concentration in the Third Molars from Residents of the Legnica-Głogów Copper Area and Risk Factors Determining the Accumulation of Those Metals: A Pilot Study

Abstract

The purpose of this study was to determine tissue concentration of toxic metals, namely Mn, Cr, Ni, Cu, Fe, Cd, Pb, and Zn, in the removed third molars in patients from the Legnica-Głogów copper district. A group of 69 patients with an average age of 27.3 ± 6.9 years was enrolled into the study. There were 16 (23.2%) men and 53 (76.8%) women. Patients were divided into two groups according to the place of residence—residents of the Legnica-Głogów Copper Area (n = 49) and the control group, residents of Wrocław (n = 20). Determination of the Pb, Cd, Cr, Ni, Fe, Mn, Cu, Zn content was performed by atomic absorption spectrometry in an air–acetylene flame using the SpectraAA atomic absorption spectrometer with a V2 AA240FS flame attachment. The content of Fe and Pb in the third molars was higher among residents of the Legnica-Głogów Copper Area (p = 0.016 and p = 0.002, respectively). The significant risk factors that may contribute to the accumulation of toxic metals in teeth were identified. We showed a significant correlation between the level of Cr, Cu, and Zn and age, and between chromium and vitamin D3 concentration in the blood (p < 0.05 for all).

Keywords

• toxic metals
• third molars
• vitamin D3
• biomonitoring
• elements