microRNA-214-mediated UBC9 expression in glioma

Abstract

It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9),the unique enzyme2 in the sumoylation pathway, is up-regulatedin many cancers. However, the expression and regulation ofUBC9 in glioma remains unknown. In this study, we found thatUbc9 was up-regulated in glioma tissues and cell lines comparedto a normal control. UBC9 knockdown by small interfering RNA(siRNA) affected cell proliferation and apoptosis in T98G cells.Further experiments revealed that microRNA (miR)-214 directlytargeted the 3' untranslated region (UTR) of UBC9 and that therewas an inverse relationship between the expression levels ofmiR-214 and UBC9 protein in glioma tissues and cells. miR-214overexpression suppressed the endogenous UBC9 protein andaffected T98G cell proliferation. These findings suggest thatmiR-214 reduction facilitates UBC9 expression and is involvedin the regulation of glioma cell proliferation

Keywords

• Cell proliferation
• Glioma
• miR-214
• Sumoylation
• UBC9