Development and Characterization of a Highly Sensitive NanoLuciferase-Based Immunoprecipitation System for the Detection of Anti-Influenza Virus HA Antibodies
Tomoko Honda,
Sumiko Gomi,
Daisuke Yamane,
Fumihiko Yasui,
Takuya Yamamoto,
Tsubasa Munakata,
Yasushi Itoh,
Kazumasa Ogasawara,
Takahiro Sanada,
Kenzaburo Yamaji,
Yasuhiro Yasutomi,
Kyoko Tsukiyama-Kohara,
Michinori Kohara
Affiliations
Tomoko Honda
Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Sumiko Gomi
Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Daisuke Yamane
Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Fumihiko Yasui
Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Takuya Yamamoto
Laboratory of Immunosenescence, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan
Tsubasa Munakata
Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Influenza virus HA-specific antibodies can be detected via the hemagglutination inhibition (HI) assay, the neutralization (NT) assay, and the enzyme-linked immunosorbent assay (ELISA). However, these assays have some drawbacks, including narrow dynamic range and the requirement for large amounts of sera.
