eLife (Nov 2020)
The neural basis for a persistent internal state in Drosophila females
- David Deutsch,
- Diego Pacheco,
- Lucas Encarnacion-Rivera,
- Talmo Pereira,
- Ramie Fathy,
- Jan Clemens,
- Cyrille Girardin,
- Adam Calhoun,
- Elise Ireland,
- Austin Burke,
- Sven Dorkenwald,
- Claire McKellar,
- Thomas Macrina,
- Ran Lu,
- Kisuk Lee,
- Nico Kemnitz,
- Dodam Ih,
- Manuel Castro,
- Akhilesh Halageri,
- Chris Jordan,
- William Silversmith,
- Jingpeng Wu,
- H Sebastian Seung,
- Mala Murthy
Affiliations
- David Deutsch
- ORCiD
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Diego Pacheco
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Lucas Encarnacion-Rivera
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Talmo Pereira
- ORCiD
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Ramie Fathy
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Jan Clemens
- ORCiD
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Cyrille Girardin
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Adam Calhoun
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Elise Ireland
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Austin Burke
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Sven Dorkenwald
- Princeton Neuroscience Institute, Princeton University, Princeton, United States; Department of Computer Science, Princeton University, Princeton, United States
- Claire McKellar
- ORCiD
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Thomas Macrina
- Princeton Neuroscience Institute, Princeton University, Princeton, United States; Department of Computer Science, Princeton University, Princeton, United States
- Ran Lu
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Kisuk Lee
- Princeton Neuroscience Institute, Princeton University, Princeton, United States; Brain & Cognitive Science Department, Massachusetts Institute of Technology, Cambridge, United States
- Nico Kemnitz
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Dodam Ih
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Manuel Castro
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Akhilesh Halageri
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Chris Jordan
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- William Silversmith
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- Jingpeng Wu
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- H Sebastian Seung
- Princeton Neuroscience Institute, Princeton University, Princeton, United States; Department of Computer Science, Princeton University, Princeton, United States
- Mala Murthy
- ORCiD
- Princeton Neuroscience Institute, Princeton University, Princeton, United States
- DOI
- https://doi.org/10.7554/eLife.59502
- Journal volume & issue
-
Vol. 9
Abstract
Sustained changes in mood or action require persistent changes in neural activity, but it has been difficult to identify the neural circuit mechanisms that underlie persistent activity and contribute to long-lasting changes in behavior. Here, we show that a subset of Doublesex+ pC1 neurons in the Drosophila female brain, called pC1d/e, can drive minutes-long changes in female behavior in the presence of males. Using automated reconstruction of a volume electron microscopic (EM) image of the female brain, we map all inputs and outputs to both pC1d and pC1e. This reveals strong recurrent connectivity between, in particular, pC1d/e neurons and a specific subset of Fruitless+ neurons called aIPg. We additionally find that pC1d/e activation drives long-lasting persistent neural activity in brain areas and cells overlapping with the pC1d/e neural network, including both Doublesex+ and Fruitless+ neurons. Our work thus links minutes-long persistent changes in behavior with persistent neural activity and recurrent circuit architecture in the female brain.
Keywords
