Effects of Resistance Exercise Training on Leptin Receptor (Ob-R), GLUT2 mRNA of the Hypothalamus and GLUT4 mRNA Expression of the Skeletal Muscle in OLETF Rats

Abstract

PURPOSE The hypothalamus plays a central role in integrating hormonal and nutritional signals from the periphery and modulating food intake, energy balance. However, obesity and type 2 diabetes cause dysfunction of hypothalamus. This study was to investigate the effects of 8 weeks resistance exercise training on leptin receptor, GLUT2 mRNA of the hypothalamus and skeletal muscle GLUT4 mRNA expression in OLETF rats. METHODS Thirty male OLETF rats were randomly divided into three groups: LETO (CON, n=10), OLETF (OLETF+CON, n=10), OLETF+resistance exercise (OLETF+EXE, n=10). Exercise group conducted gradual ladder climbing exercise 3 days per week for 8 weeks. RESULTS The results of this study was following; In the hypothalamus, leptin receptor (Ob-R) and GLUT2 mRNA expression were significantly increased in OLETF+EXE group compared with OLETF group (p<.05). Activation of AMPK was significantly decreased in OLETF+EXE group compared to OLETF group (p<.05). GLUT4 mRNA expression of skeletal muscle was significantly increased in OLETF+EXE group compared with OLETF group (p<.05), and HOMA-IR index also was significantly lower in OLETF+EXE group than OLETF group (p<.05). CONCLUSIONS These results showed that 8 weeks resistance exercise training can improve leptin receptor mRNA expression and glucose sensing in hypothalamic. Also, these effects may contribute to energy homeostasis in peripheral such as skeletal muscle.

Keywords

• resistance exercise
• hypothalamus
• type 2 diabetes mellitus
• leptin receptor (ob-r)
• gluts