Multiplexed characterization of rationally designed promoter architectures deconstructs combinatorial logic for IPTG-inducible systems

Timothy C. Yu; Winnie L. Liu; Marcia S. Brinck; Jessica E. Davis; Jeremy Shek; Grace Bower; Tal Einav; Kimberly D. Insigne; Rob Phillips; Sriram Kosuri; Guillaume Urtecho

doi:10.1038/s41467-020-20094-3

Nature Communications (Jan 2021)

Multiplexed characterization of rationally designed promoter architectures deconstructs combinatorial logic for IPTG-inducible systems

Timothy C. Yu,
Winnie L. Liu,
Marcia S. Brinck,
Jessica E. Davis,
Jeremy Shek,
Grace Bower,
Tal Einav,
Kimberly D. Insigne,
Rob Phillips,
Sriram Kosuri,
Guillaume Urtecho

Affiliations

Timothy C. Yu: Department of Bioengineering, University of California
Winnie L. Liu: Department of Molecular, Cell, and Developmental Biology, University of California
Marcia S. Brinck: Department of Microbiology, Immunology, and Molecular Genetics, University of California
Jessica E. Davis: Department of Chemistry and Biochemistry, University of California
Jeremy Shek: Department of Chemistry and Biochemistry, University of California
Grace Bower: Department of Molecular, Cell, and Developmental Biology, University of California
Tal Einav: Department of Physics, California Institute of Technology
Kimberly D. Insigne: Bioinformatics Interdepartmental Graduate Program, University of California
Rob Phillips: Department of Physics, California Institute of Technology
Sriram Kosuri: Department of Chemistry and Biochemistry, University of California
Guillaume Urtecho: Molecular Biology Interdepartmental Doctoral Program, University of California

DOI: https://doi.org/10.1038/s41467-020-20094-3
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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Precisely tuning the genetic response to environmental stimuli is a key step in engineering synthetic biology systems. Here, the authors profile 8269 IPTG-induced promoters to deconstruct the relationship between sequence architecture and gene expression.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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