Allosteric modulation of LRRC8 channels by targeting their cytoplasmic domains

Dawid Deneka; Sonja Rutz; Cedric A. J. Hutter; Markus A. Seeger; Marta Sawicka; Raimund Dutzler

doi:10.1038/s41467-021-25742-w

Nature Communications (Sep 2021)

Allosteric modulation of LRRC8 channels by targeting their cytoplasmic domains

Dawid Deneka,
Sonja Rutz,
Cedric A. J. Hutter,
Markus A. Seeger,
Marta Sawicka,
Raimund Dutzler

Affiliations

Dawid Deneka: Department of Biochemistry University of Zurich
Sonja Rutz: Department of Biochemistry University of Zurich
Cedric A. J. Hutter: Institute of Medical Microbiology University of Zurich
Markus A. Seeger: Institute of Medical Microbiology University of Zurich
Marta Sawicka: Department of Biochemistry University of Zurich
Raimund Dutzler: Department of Biochemistry University of Zurich

DOI: https://doi.org/10.1038/s41467-021-25742-w
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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Volume-regulated anion channels (VRACs) are heteromers of LRRC8 proteins, all containing the obligatory subunit LRRC8A. Here, the authors develop and characterize nanobodies that bind LRRC8A and allosterically modulate the function of homomeric LRRC8A and endogenous heteromeric channels, hinting at functional mechanisms present in VRACs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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