Subcellular analysis of blood-brain barrier function by micro-impalement of vessels in acute brain slices

Amira Sayed Hanafy; Pia Steinlein; Julika Pitsch; Mariella Hurtado Silva; Natascha Vana; Albert J. Becker; Mark Evan Graham; Susanne Schoch; Alf Lamprecht; Dirk Dietrich

doi:10.1038/s41467-023-36070-6

Nature Communications (Jan 2023)

Subcellular analysis of blood-brain barrier function by micro-impalement of vessels in acute brain slices

Amira Sayed Hanafy,
Pia Steinlein,
Julika Pitsch,
Mariella Hurtado Silva,
Natascha Vana,
Albert J. Becker,
Mark Evan Graham,
Susanne Schoch,
Alf Lamprecht,
Dirk Dietrich

Affiliations

Amira Sayed Hanafy: Department of Neurosurgery, University Hospital Bonn
Pia Steinlein: Department of Neurosurgery, University Hospital Bonn
Julika Pitsch: Section for Translational Epilepsy Research, Dept. of Neuropathology, University Hospital Bonn
Mariella Hurtado Silva: Synapse Proteomics, Children’s Medical Research Institute, The University of Sydney
Natascha Vana: Department of Neurosurgery, University Hospital Bonn
Albert J. Becker: Section for Translational Epilepsy Research, Dept. of Neuropathology, University Hospital Bonn
Mark Evan Graham: Synapse Proteomics, Children’s Medical Research Institute, The University of Sydney
Susanne Schoch: Section for Translational Epilepsy Research, Dept. of Neuropathology, University Hospital Bonn
Alf Lamprecht: Department of Pharmaceutics, Institute of Pharmacy, University of Bonn
Dirk Dietrich: Department of Neurosurgery, University Hospital Bonn

DOI: https://doi.org/10.1038/s41467-023-36070-6
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 19

Abstract

Read online

The blood-brain barrier (BBB) tightly and dynamically regulates exchange with the brain. Here, the authors report a brain slice approach to study the native BBB in a highly controlled manner by combining intravascular perfusion and multiphoton microscopy.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal