Cancer Management and Research (May 2021)
Investigation of Chromosome 1 Aberrations in the Lymphocytes of Prostate Cancer and Benign Prostatic Hyperplasia Patients by Fluorescence in situ Hybridization
Abstract
Justyna Miszczyk,1 Mikołaj Przydacz,2 Michał Zembrzuski,2 Piotr L Chłosta2 1Department of Experimental Physics of Complex Systems, The H. Niewodniczański Institute of Nuclear Physics PAN, Krakow, Poland; 2Department of Urology, Jagiellonian University Medical College, Krakow, PolandCorrespondence: Justyna MiszczykDepartment of Experimental Physics of Complex Systems, The H. Niewodniczański Institute of Nuclear Physics PAN, Radzikowskiego 152 Street, Krakow, 31-342, PolandTel +48 126628409Fax +48 126628458Email [email protected]: Radiotherapy is one of the most common treatments for prostate cancer. Finding a useful predictor of the therapeutic outcome is crucial as it increases the efficacy of treatment planning. This study investigated the individual susceptibility to radiation based on chromosome 1 aberration frequency measured by the FISH (fluorescence in situ hybridization) method.Patients and Methods: Whole blood samples were collected from 27 prostate cancer (PCa) patients and 32 subjects with benign prostatic hyperplasia (BPH), who were considered as a control group. Samples were irradiated with 2 Gy of x-rays, cultured, harvested, and used in the FISH procedure.Results: After irradiation, significantly higher levels of all studied chromosome 1 aberrations (except for deletions) in the group of PCa patients were revealed. Furthermore, in the lymphocytes of cancer patients, nearly five-fold higher frequencies of acentric fragments were observed compared to the BPH group. The highest individual radiosensitivities for all estimated biomarkers were seen in PCa patient cells who reported cancer incidence in the immediate family (CIF+).Conclusion: The differences in chromosome 1 aberrations between PCa and BPH demonstrate that lymphocytes taken from patients with prostate cancer have higher radiosensitivity which might be related to hereditary or familiar inclinations. Therefore, this technique may find future application in searching biomarkers of the cellular radiotherapy response in prostate cancer patients.Keywords: benign prostatic hyperplasia, chromosome 1, prostate cancer, radiotherapy planning, biomarkers, FISH method
