Effect of different inoculum preparation conditions on the biomass growth and antimicrobial activity of Bacillus sp.

Abstract

Plant diseases caused by pathogenic bacteria and fungi can lead to reduced plant growth capacity and significant losses in food production. The use of biopesticides is strongly recommended over applying chemical compounds due to growing concerns about environmental pollution and potential harmful effects to human health. A significant scientific and economic interest lies in the isolation of new Bacillus strains showing the potential for plant disease biocontrol. The purpose of this study is to investigate the effect of different inoculum preparation conditions on the Bacillus sp. biomass growth and antimicrobial activity against the selected phytopathogenic Xanthomonas strains. The results obtained argue the possibility of utilizing the glycerol-based semi-synthetic medium in the second stage of inoculum preparation and reducing the duration of inoculum preparation, which is of great importance to the bioprocess cost attenuation and the development of optimized bioprocess solutions for producing bioactive agents effective against the phytopathogenic Xanthomonas strains.

Keywords

• bacillus sp.
• biomass
• inoculum
• antimicrobial activity
• xanthomonas