The Effect of TGF-alpha on Neurogenesis in Subventricular Zone of Rat Brain after Ischemia-Reperfusion

Abstract

Introduction: Stroke is the third important reason of death in adults and an important cause of adult disability. Previous studies suggest that TGF-alpha can induce neurogenesis after stroke. Here in, we studied neurogenesis effects of the TGF-alpha on subventricular zone following ischemia-reperfusion. Male wistar rats (250-300 g) were divided into ischemia and treatment groups. After induction of ischemia-reperfusions, PBS (phosphate buffer salin) and TGF-alpha 50 ng were injected stereotaxicaly in lateral ventricle in ischemia and treatment respectively. After 12 days, the nestin expression in subventricular zone was assessed by immunohistochemical staining method. Results: Our results showed that nestin expression increased significantly in treatment group in comparison with ischemia group (p<0.05) . Discussion: Expression of nestin in SVZ indicates that TGF- α can stimulate the neural stem cells proliferation after ischemia –reperfusion injury. Methods:

Keywords

• Hippocampus
• Ischemia
• Subventricular Zone
• TGF-Alpha