In vitro Cell Proliferation Assay of Demineralized Dentin Material Membrane in Osteoblastic MC3T3-E1 Cells

Abstract

Pratiwi Soesilawati,1– 4 Andra Rizqiawan,4,5 Retno Indrawati Roestamadji,1,3 Ahmad Rizal Arrosyad,6 Muhammad Alwino Bayu Firdauzy,3,6 Noor Hayaty Abu Kasim7,8 1Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; 2Cell and Tissue Bank-Regenerative Medicine, Dr Soetomo General Academic Hospital/Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; 3Immunology Program, Postgraduate School, Universitas Airlangga, Surabaya, Indonesia; 4Dental Hospital, Universitas Airlangga, Surabaya, Indonesia; 5Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; 6Dental Profession Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; 7Faculty of Dentistry, University Kebangsaan Malaysia, Kuala Lumpur, Malaysia; 8Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaCorrespondence: Pratiwi SoesilawatiDepartment of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Jl Mayjend Prof. Dr. Moestopo No. 47, Surabaya, 60132, IndonesiaTel [phone]Fax [phone]Email [email protected]: Demineralized dentin material membrane (DDMM) is a novel bioresorbable guided bone regeneration (GBR) which is derived from the demineralization process of bovine dentin. This material/process could be an alternative to resolve musculoskeletal dysfunction that harms the quality of human life.Purpose: To evaluate the cytotoxic effect of DDMM as GBR membrane on MC3T3-E1 osteoblast cell line.Methods: Cytotoxic effect was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Osteoblast MC3T3-E1 cell culture was used as a parameter of cell viability after reacting with GBR materials. The absorbance values were examined at each treatment to determine the percentage of cell viability. There were four groups created in the present study: two treatment groups and two control groups. The treatment groups consisted of a DDMM group and a bovine pericardium collagen membrane (BPCM) group. The control groups comprised a group containing cell culture medium as a negative control group and another positive control group that contained cell cultures.Results: The results revealed no significant difference in MC3T3-E1 cell viability between the treatment and control groups (p < 0.05). Moreover, as observed in the DDMM group, there was an increase in the number of osteoblast cells.Conclusion: DDMM is a suitable alternative biomaterial for GBR as it is non-cytotoxic and could potentially increase the rate of repair of craniofacial defects.Keywords: cells, biomedical and dental materials, oral surgical procedures, materials testing, wound, injuries, cytotoxicity test, pre-prosthetic

Keywords

• cells
• biomedical and dental materials
• oral surgical procedures
• materials testing
• wound
• injuries
• cytotoxicity test
• pre-prosthetic