Polyinosinic: Polycytidylic Acid and Murine Cytomegalovirus Modulate Expression of Murine IL-10 and IL-21 in White Adipose Tissue

Pablo Garcia-Valtanen; Ruth Marian Guzman-Genuino; John D. Hayball; Kerrilyn R. Diener

doi:10.3390/v12050569

Viruses (May 2020)

Polyinosinic: Polycytidylic Acid and Murine Cytomegalovirus Modulate Expression of Murine IL-10 and IL-21 in White Adipose Tissue

Pablo Garcia-Valtanen,
Ruth Marian Guzman-Genuino,
John D. Hayball,
Kerrilyn R. Diener

Affiliations

Pablo Garcia-Valtanen: Experimental Therapeutics Laboratory, UniSA Cancer Research Institute, Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia
Ruth Marian Guzman-Genuino: Experimental Therapeutics Laboratory, UniSA Cancer Research Institute, Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia
John D. Hayball: Experimental Therapeutics Laboratory, UniSA Cancer Research Institute, Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia
Kerrilyn R. Diener: Experimental Therapeutics Laboratory, UniSA Cancer Research Institute, Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia

DOI: https://doi.org/10.3390/v12050569
Journal volume & issue: Vol. 12, no. 5
p. 569

Abstract

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White adipose tissue (WAT) produces interleukin-10 and other immune suppressors in response to pathogen-associated molecular patterns (PAMPs). It also homes a subset of B-cells specialized in the production of IL-10, referred to as regulatory B-cells. We investigated whether viral stimuli, polyinosinic: polycytidylic acid (poly(I:C)) or whole replicative murine cytomegalovirus (MCMV), could stimulate the expression of IL-10 in murine WAT using in vivo and ex vivo approaches. Our results showed that in vivo responses to systemic administration of poly(I:C) resulted in high levels of endogenously-produced IL-10 and IL-21 in WAT. In ex vivo WAT explants, a subset of B-cells increased their endogenous IL-10 expression in response to poly(I:C). Finally, MCMV replication in WAT explants resulted in decreased IL-10 levels, opposite to the effect seen with poly(I:C). Moreover, downregulation of IL-10 correlated with relatively lower number of Bregs. To our knowledge, this is the first report of IL-10 expression by WAT and WAT-associated B-cells in response to viral stimuli.

Published in Viruses

ISSN: 1999-4915 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.mdpi.com/journal/viruses

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