Einstein (São Paulo) ()

Urinary beta-trace protein gene expression analysis in type 2 diabetes mellitus patients

  • Marcelo Rodrigues Bacci,
  • Beatriz da Costa Aguiar Alves,
  • Marina Romera Cavallari,
  • Ligia Ajaime Azzalis,
  • Ross Martin de Rozier-Alves,
  • Matheus Moreira Perez,
  • Ethel Zimberg Chehter,
  • Edimar Cristiano Pereira,
  • Fernando Luiz Affonso Fonseca

DOI
https://doi.org/10.1590/s1679-45082017ao4012
Journal volume & issue
Vol. 15, no. 4
pp. 441 – 444

Abstract

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ABSTRACT Objective: To evaluate the gene expression of beta-trace protein in urine of diabetic patients, with no reduction in glomerular filtration rate, which was defined as below 60mL/min/1.73m2. Methods: Type 2 diabetes mellitus patients were recruited, and a group of non-diabetic individuals served as control. Beta-trace protein gene expression was analyzed by quantitative PCR. Blood samples were collected to establish glucose levels and baseline kidney function. Accuracy was analyzed using ROC curves. Results: Ninety type 2 diabetes mellitus patients and 20 non-diabetic individuals were recruited. The area under the curve was 0.601, sensitivity of 20% and specificity of 89.47%. Among diabetic participants, 18% showed an expression above the cutoff point. Conclusion: These results of accuracy of beta-trace protein gene expression in urine of diabetic patients are promising, although they did not achieve a higher area under the curve level.

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