Arquivo Brasileiro de Medicina Veterinária e Zootecnia (Dec 2014)

Identificação de loci microssatélitescom potencial de amplificação na espécie de Peixe-Rei (Odontesthes humensis)

  • R.A. Tavares,
  • S.R.N. Piedras,
  • M.D. Nunes,
  • D.B. Almeida,
  • C.G.A. Moreira,
  • J.M. Fernandes,
  • S.F. Freitas,
  • H.L.M. Moreira,
  • J.L.O.F. Pouey,
  • N.J.L. Dionello

DOI
https://doi.org/10.1590/1678-7444
Journal volume & issue
Vol. 66, no. 6
pp. 1941 – 1945

Abstract

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In this work, 25,806 potentially amplifiable microsatellite loci (PAL) were identified in pejerrey, (Odontesthes humensis), with 21% of dinucleotide, 22% trinucleotide, 37% tetranucleotide, 13% pentanucleotide and 7% hexanucleotide. Of the total loci, 167 were classified as "Best PAL", more likely to be variables in populations. The results show that with a small coverage of the genome it was possible to identify a large number of microsatellite loci.

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