Stem Cell Research (Sep 2024)
Generation of a gene-corrected human isogenic iPSC line from an Alzheimer’s disease iPSC line carrying the PSEN1 H163R mutation
- Damián Hernández,
- Stephanie Morgan Schlicht,
- Jordan Elli Clarke,
- Maciej Daniszewski,
- Celeste M. Karch,
- Alison M. Goate,
- Alice Pébay,
- Sarah Adams,
- Ricardo Allegri,
- Aki Araki,
- Nicolas Barthelemy,
- Randall Bateman,
- Jacob Bechara,
- Tammie Benzinger,
- Sarah Berman,
- Courtney Bodge,
- Sus Brandon,
- William (Bill) Brooks,
- Jared Brosch,
- Jill Buck,
- Virginia Buckles,
- Kathleen Carter,
- Lisa Cash,
- Charlie Chen,
- Jasmeer Chhatwal,
- Patricio Chrem Mendez,
- Jasmin Chua,
- Helena Chui,
- Laura Courtney,
- Carlos Cruchaga,
- Gregory S Day,
- Chrismary DeLaCruz,
- Darcy Denner,
- Anna Diffenbacher,
- Aylin Dincer,
- Tamara Donahue,
- Jane Douglas,
- Duc Duong,
- Noelia Egido,
- Bianca Esposito,
- Anne Fagan,
- Marty Farlow,
- Becca Feldman,
- Colleen Fitzpatrick,
- Shaney Flores,
- Nick Fox,
- Erin Franklin,
- Nelly Joseph-Mathurin,
- Hisako Fujii,
- Samantha Gardener,
- Bernardino Ghetti,
- Alison Goate,
- Sarah Goldberg,
- Jill Goldman,
- Alyssa Gonzalez,
- Brian Gordon,
- Susanne Gr¨aber-Sultan,
- Neill Graff-Radford,
- Morgan Graham,
- Julia Gray,
- Emily Gremminger,
- Miguel Grilo,
- Alex Groves,
- Christian Haass,
- Lisa H¨asler,
- Jason Hassenstab,
- Cortaiga Hellm,
- Elizabeth Herries,
- Laura Hoechst-Swisher,
- Anna Hofmann,
- Anna Hofmann,
- David Holtzman,
- Russ Hornbeck,
- Yakushev Igor,
- Ryoko Ihara,
- Takeshi Ikeuchi,
- Snezana Ikonomovic,
- Kenji Ishii,
- Clifford Jack,
- Gina Jerome,
- Erik Johnson,
- Mathias Jucker,
- Celeste Karch,
- Stephan K¨aser,
- Kensaku Kasuga,
- Sarah Keefe,
- William Klunk,
- Robert Koeppe,
- Deb Koudelis,
- Elke Kuder-Buletta,
- Christoph Laske,
- Allan Levey,
- Johannes Levin,
- Yan Li,
- Oscar Lopez,
- Jacob Marsh,
- Ralph Martins,
- Neal Scott Mason,
- Colin Masters,
- Kwasi Mawuenyega,
- Austin McCullough,
- Eric McDade,
- Arlene Mejia,
- Estrella Morenas-Rodriguez,
- John Morris,
- James Mountz,
- Cath Mummery,
- Neelesh Nadkarni,
- Akemi Nagamatsu,
- Katie Neimeyer,
- Yoshiki Niimi,
- James Noble,
- Joanne Norton,
- Brigitte Nuscher,
- Ulricke Obermüller,
- Antoinette O’Connor,
- Riddhi Patira,
- Richard Perrin,
- Lingyan Ping,
- Oliver Preische,
- Alan Renton,
- John Ringman,
- Stephen Salloway,
- Peter Schofield,
- Michio Senda,
- Nicholas T Seyfried,
- Kristine Shady,
- Hiroyuki Shimada,
- Wendy Sigurdson,
- Jennifer Smith,
- Lori Smith,
- Beth Snitz,
- Hamid Sohrabi,
- Sochenda Stephens,
- Kevin Taddei,
- Sarah Thompson,
- Jonathan V¨oglein,
- Peter Wang,
- Qing Wang,
- Elise Weamer,
- Chengjie Xiong,
- Jinbin Xu,
- Xiong Xu
Affiliations
- Damián Hernández
- Department of Anatomy and Physiology, the University of Melbourne, Parkville, VIC 3010, Australia
- Stephanie Morgan Schlicht
- Department of Anatomy and Physiology, the University of Melbourne, Parkville, VIC 3010, Australia
- Jordan Elli Clarke
- Department of Anatomy and Physiology, the University of Melbourne, Parkville, VIC 3010, Australia
- Maciej Daniszewski
- Department of Anatomy and Physiology, the University of Melbourne, Parkville, VIC 3010, Australia
- Celeste M. Karch
- Department of Psychiatry, Washington University School of Medicine, St. Louis, MO 63110, USA
- Alison M. Goate
- Department of Genetics and Genomic Sciences, Ronald M. Loeb Center for Alzheimer’s Disease, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USA
- Alice Pébay
- Department of Anatomy and Physiology, the University of Melbourne, Parkville, VIC 3010, Australia; Department of Surgery, Royal Melbourne Hospital, the University of Melbourne, Parkville, VIC 3010, Australia; Corresponding author.
- Sarah Adams
- Ricardo Allegri
- Aki Araki
- Nicolas Barthelemy
- Randall Bateman
- Jacob Bechara
- Tammie Benzinger
- Sarah Berman
- Courtney Bodge
- Sus Brandon
- William (Bill) Brooks
- Jared Brosch
- Jill Buck
- Virginia Buckles
- Kathleen Carter
- Lisa Cash
- Charlie Chen
- Jasmeer Chhatwal
- Patricio Chrem Mendez
- Jasmin Chua
- Helena Chui
- Laura Courtney
- Carlos Cruchaga
- Gregory S Day
- Chrismary DeLaCruz
- Darcy Denner
- Anna Diffenbacher
- Aylin Dincer
- Tamara Donahue
- Jane Douglas
- Duc Duong
- Noelia Egido
- Bianca Esposito
- Anne Fagan
- Marty Farlow
- Becca Feldman
- Colleen Fitzpatrick
- Shaney Flores
- Nick Fox
- Erin Franklin
- Nelly Joseph-Mathurin
- Hisako Fujii
- Samantha Gardener
- Bernardino Ghetti
- Alison Goate
- Sarah Goldberg
- Jill Goldman
- Alyssa Gonzalez
- Brian Gordon
- Susanne Gr¨aber-Sultan
- Neill Graff-Radford
- Morgan Graham
- Julia Gray
- Emily Gremminger
- Miguel Grilo
- Alex Groves
- Christian Haass
- Lisa H¨asler
- Jason Hassenstab
- Cortaiga Hellm
- Elizabeth Herries
- Laura Hoechst-Swisher
- Anna Hofmann
- Anna Hofmann
- David Holtzman
- Russ Hornbeck
- Yakushev Igor
- Ryoko Ihara
- Takeshi Ikeuchi
- Snezana Ikonomovic
- Kenji Ishii
- Clifford Jack
- Gina Jerome
- Erik Johnson
- Mathias Jucker
- Celeste Karch
- Stephan K¨aser
- Kensaku Kasuga
- Sarah Keefe
- William Klunk
- Robert Koeppe
- Deb Koudelis
- Elke Kuder-Buletta
- Christoph Laske
- Allan Levey
- Johannes Levin
- Yan Li
- Oscar Lopez
- Jacob Marsh
- Ralph Martins
- Neal Scott Mason
- Colin Masters
- Kwasi Mawuenyega
- Austin McCullough
- Eric McDade
- Arlene Mejia
- Estrella Morenas-Rodriguez
- John Morris
- James Mountz
- Cath Mummery
- Neelesh Nadkarni
- Akemi Nagamatsu
- Katie Neimeyer
- Yoshiki Niimi
- James Noble
- Joanne Norton
- Brigitte Nuscher
- Ulricke Obermüller
- Antoinette O’Connor
- Riddhi Patira
- Richard Perrin
- Lingyan Ping
- Oliver Preische
- Alan Renton
- John Ringman
- Stephen Salloway
- Peter Schofield
- Michio Senda
- Nicholas T Seyfried
- Kristine Shady
- Hiroyuki Shimada
- Wendy Sigurdson
- Jennifer Smith
- Lori Smith
- Beth Snitz
- Hamid Sohrabi
- Sochenda Stephens
- Kevin Taddei
- Sarah Thompson
- Jonathan V¨oglein
- Peter Wang
- Qing Wang
- Elise Weamer
- Chengjie Xiong
- Jinbin Xu
- Xiong Xu
- Journal volume & issue
-
Vol. 79
p. 103495
Abstract
We report the generation of a gene-edited human induced pluripotent stem cell (iPSC) line from an Alzheimer’s disease patient-derived iPSC line harbouring the PSEN1 H163R mutation. This line demonstrates pluripotent stem cell morphology, expression of pluripotency markers, and maintains a normal karyotype.