Complete chloroplast genome sequence of Adenostemma madurense (Asteraceae)

Abstract

Adenostemma madurense D.C. belongs to the family Asteraceae and is a wild annual herb found in Korea. In this study, we determined the phylogenetic position of A. madurense by identifying its complete chloroplast sequence. The result indicated a genome size of 150,054 bp, which was composed of a large single copy of 82,008 bp, two inverted repeats of 24,952 bp each, and a small singlecopy of 18,142 bp. Overall, 128 unique genes were identified, including 85 protein-coding, 35 tRNA, and 8 rRNA genes. A comparison of the A. madurense and A. lavenia chloroplast genomes revealed seven variations. Phylogenetic analysis revealed that A. madurense formed a clade with A. lavenia and had a well-supported phylogenetic relationship with Asteraceae. The first complete chloroplast genome of A. madurense was obtained, which would be helpful in explaining the process of speciation in Adenostemma and the development of molecular markers.

Keywords

• Adenostemma madurense
• Asteraceae
• chloroplast genome
• phylogenetic relationship