The comparative effects of probiotics on growth, antioxidant indices and intestinal histomorphology of broilers under heat stress condition

Abstract

Abstract Heat stress adversely affects both the productivity and well-being of chickens. Probiotics offer beneficial impacts on the health and growth performance of broilers. The current study investigates the influence of administering of Bacillus (including B. subtilis, B. licheniformis, B. coagulans, and B. indicus) and Lactobacillus (consisting of L. acidophilus, L. plantarum, L. buchneri, and L. rhamnosus) probiotics via drinking water, either singular or combined, on various aspects including growth performance, oxidative stress markers, carcass characteristics, fecal microbial composition, intestinal structure, and intestinal pH in broilers exposed to chronic heat stress. A total of 150 one-day-old broiler chicks were divided into 5 groups: (1) NC, negative control; (2) HS, birds exposed to chronic heat stress; (3) HSpBacil, exposed to chronic heat stress and received Bacillus probiotic; (4) HSpLAB, subjected to chronic heat stress and provided with Lactobacillus probiotic; (5) HSpMix, subjected to chronic heat stress and administered a combined probiotic from Bacillus and Lactobacillus. The HS group exhibited significantly reduced levels of growth performance, carcass traits, and notably affected oxidative stress indices, as well as intestinal pH and histomorphology in the birds. Additionally, the administered probiotics led to increased weight of lymphoid organs, enhanced body weight gain, and improved intestinal histomorphology. Furthermore, the probiotics decreased malondialdehyde and increased total antioxidant capacity in broilers. In conclusion, Bacillus and Lactobacillus probiotics, as single or multi-species, particularly Lactobacillus and combined probiotic, demonstrated potential in alleviating the adverse effects of heat stress in broiler chickens. They could serve as beneficial feed additives and growth enhancers.

Keywords

• Antioxidant indices
• Broilers
• Heat stress
• Intestinal histology
• Probiotic
• Productivity