Entourage effect for phenolic compounds on production and metabolism of mammary epithelial cells
Y. Shalev,
O. Hadaya,
R. Bransi-Nicola,
S.Y. Landau,
H. Azaizeh,
H. Muklada,
T. Glasser,
Z. Roth,
T. Deutch-Traubman,
M. Haj-Zaroubi,
N. Argov-Argaman
Affiliations
Y. Shalev
Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, 7610001, Israel
O. Hadaya
Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, 7610001, Israel; Department of Natural Resources, Institute of Plant Sciences, Agricultural Research Organization – the Volcani Center, Rishon LeZion, 7505101, Israel
R. Bransi-Nicola
Institute of Applied Research, The Galilee Society, P.O. Box 437, Shefa-Amr, 20200, Israel
S.Y. Landau
Department of Natural Resources, Institute of Plant Sciences, Agricultural Research Organization – the Volcani Center, Rishon LeZion, 7505101, Israel
H. Azaizeh
Institute of Applied Research, The Galilee Society, P.O. Box 437, Shefa-Amr, 20200, Israel; Department of Natural Resources & Environmental Management, University of Haifa, Haifa, 3498838, Israel; Tel Hai College, Department of Environmental Science, Upper Galilee, 12208, Israel
H. Muklada
Department of Natural Resources, Institute of Plant Sciences, Agricultural Research Organization – the Volcani Center, Rishon LeZion, 7505101, Israel
T. Glasser
Ramat HaNadiv Nature Park, Zikhron Yaakov, Israel
Z. Roth
Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, 7610001, Israel
T. Deutch-Traubman
Department of Natural Resources, Institute of Plant Sciences, Agricultural Research Organization – the Volcani Center, Rishon LeZion, 7505101, Israel
M. Haj-Zaroubi
Institute of Applied Research, The Galilee Society, P.O. Box 437, Shefa-Amr, 20200, Israel; Department of Natural Resources & Environmental Management, University of Haifa, Haifa, 3498838, Israel
N. Argov-Argaman
Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, 7610001, Israel; Corresponding author.
Primary culture of mammary epithelial cells (MEC) was exposed to ethyl-acetate, chloroform and hexane extracts of Pistacia lentiscus (lentisk). The hexane extract contained mainly ethyl gallate whereas the chloroform extract contained mainly ethyl-gallate with smaller amount of gallic acid, and the ethyl-acetate extract contained mainly rutin, gallic acid and myricetin. Ethyl acetate extract increased secretion of protein and fat and improved mitochondrial activity. The enhancing effect on protein production was attributed to myricetin, one of the polyphenols in the ethyl-acetate extract whereas gallic acid did not affect protein production or secretion. Interestingly, exposure to the isolated polyphenols did not improve mitochondrial productivity and activity as effectively as exposure to the complete plant extract. The results indicated that polyphenols improve production of milk constituents by MEC, through different modes of action for different polyphenols suggesting an additive or even synergistic effect on production traits of mammary cells.
