Simultaneous removal of concentrated organics, nitrogen and phosphorus nutrients by an oxygen-limited membrane bioreactor.

Abstract

Simultaneous removal of organics, nitrogen and phosphorus was achieved in a bench-scale oxygen-limited membrane bioreactor (OLMBR). Due to the limited dissolved oxygen (~ 0.2 mg/L equilibrium concentration) and the increased sludge concentration associated with the hollow fiber membrane, the OLMBR was endowed with an excellent performance on the removal of multi-pollutants. The optimized removal efficiencies of COD, nitrogen (N), and total phosphorus (TP) were approximately 95.5%, 90.0% and 82.6%, respectively (COD/N/P = 500:10:1, influent loading = 5.0 kg COD·m-3·d-1, 35°C). Mass balance and bacterial community analysis indicated that the removal of organic carbon was mainly achieved by the methane production process (67.6%). Short-cut nitrification-denitrification (SCND) was observed as the primary denitrification process in the OLMBR, in which the concentrated organic compounds served as the electron donors for denitrification. Nitrosomonas was observed to be the predominant ammonium-oxidizing bacteria, while nitrite-oxidizing bacteria were almost absent in the microbial community as revealed by the high-throughput sequencing technique. In addition, Euryarchaeota and Candidatus, which were well associated with the process of denitrifying anaerobic methane oxidation, were also detected. Sludge absorption was the main route for TP removal in the OLMBR, and the production of PH3 gas also accounted for 19.4% of TP removal. This study suggested that the interception effect of hollow fiber membrane provided higher sludge concentration, therefore offering more bacteria for pollutant removal. The OLMBR can be used for simultaneous removal of highly concentrated organics and nutrients in livestock and poultry breeding wastewater.