Effect of Buddleja officinalis eye drops to inflammatory factors of lacrimal gland cells of castrated male rabbit with dry eye

Abstract

AIM:To observe the effect of different concentrations Buddleja offcinalis eye drops(BOED)to inflammatory factors of lacrimal gland cells of castrated male rabbit with dry eye.METHODS:Forty-two healthy adult New Zealand rabbits were randomly divided into 7 groups, 6 rabbits each group. Group A: blank group; Group B: model group; Group C: low concentrations BOED group; Group D: medium concentrations BOED; Group E: high concentrations BOED; Group F: the placebo group; Group G: testosterone groups. All rabbits of groups B to G were cut off bilateral testis and epididymis except group A. The rabbits of groups C, D, E and F were dropped relevant eye drops except groups A and B. All rabbits were tested for Schimer I test(SIT)and break-up time(BUT)before operation and 4wk after operation. Expressions of inflammatory factors of TNF-α, IL-1β from lacrimal gland cells were checked in immunohistochemical staining method.RESULTS:1)Comparison of SIT and BUT: Compared with the groups D, E and G, group C had statistical significance(PP>0.05); 2)Comparison of inflammatory factors of TNF-α, IL-1β after operation: Compared with value of the average optical density of TNF-α and IL-1β of group C, those of groups D, E and G were obviously different after operation(PP>0.05).CONCLUSION: 1)Compared with androgen, BOED has the similar effect of depressing the expression of inflammatory factors. But its effect is slightly weaker than the androgen; 2)The depressing effect of medium and high concentration BOED are better than low concentration. Compared with medium and high concentration of BOED, there was no significant difference between two groups.

Keywords

• Buddleja offcinalis eye drops; dry eye; lacrimal gland cell; inflammatory factors
• Buddleja offcinalis eye drops
• dry eye
• lacrimal gland cell
• inflammatory factors