OncoTargets and Therapy (Aug 2019)

lncRNA DSCAM-AS1 downregulates miR-216b to promote the migration and invasion of colorectal adenocarcinoma cells

  • Liu F,
  • Jia J,
  • Sun L,
  • Yu Q,
  • Duan H,
  • Jiao D,
  • Gong Z,
  • Zhu S,
  • Jiang K,
  • He Y,
  • Chen L,
  • Zhang Y,
  • Sun H

Journal volume & issue
Vol. Volume 12
pp. 6789 – 6795

Abstract

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Fei Liu, Jianhui Jia, Liping Sun, Qingrui Yu, Hongyan Duan, Dexin Jiao, Zhiqiang Gong, Shendong Zhu, Kexin Jiang, Yijiang He, Liping Chen, Yanni Zhang, Han SunDepartment of Radiotherapy, Liaoning Cancer Hospital & Institute, Liaoning City, Shenyang Province 110042, People’s Republic of ChinaCorrespondence: Fei LiuDepartment of Radiotherapy, Liaoning Cancer Hospital & Institute, No. 44 Xiaoheyan Road, Dadong District, Liaoning City, Shenyang Province 110042, People’s Republic of ChinaTel +86 243 193 6422Email [email protected]: It has been reported that lncRNA DSCAM-AS1 plays an oncogenic role in breast cancer. In the present study we explored the role of DSCAM-AS1 in colorectal adenocarcinoma (CRA).Methods: Gene expression was analyzed by qPCR and Western blot. Overexpression experiments were performed to analyze gene interactions. Transwell assays were performed to analyze cell invasion and migration. Methylation-specific PCR (MSP) was performed to analyze DNA methylation.Results: It was observed that DSCAM-AS1 was upregulated in the primary tumor tissues than in paired non-tumor tissues (within 2 cm around tumors) and was further increased with tumor metastasis. miR-216b was downregulated in primary tumor and further downregulated with tumor metastasis. miR-216b was inversely correlated with DSCAM-AS1 in tumor tissues, but not in non-tumor tissues. In cells of CRA cell lines, DSCAM-AS1 overexpression resulted in the downregulation of miR-216b, while miR-216b overexpression did not significantly affect DSCAM-AS1. DSCAM-AS1 overexpression did not significantly affect cancer cell proliferation but promoted cell migration and invasion. miR-216b inhibited cancer cell migration and invasion and significantly reduced the effects of DSCAM-AS1 overexpression. Methylation-specific PCR showed that DSCAM-AS1 overexpression promoted the methylation of miR-216b gene.Conclusion: DSCAM-AS1 may downregulate miR-216b to promote the migration and invasion of CRA cells.Keywords: colorectal adenocarcinoma, lncRNA DSCAM-AS1, miR-216b

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