Electrospun Fibers and Sorbents as a Possible Basis for Effective Composite Wound Dressings
Alan Saúl Álvarez-Suárez,
Syed G. Dastager,
Nina Bogdanchikova,
Daniel Grande,
Alexey Pestryakov,
Juan Carlos García-Ramos,
Graciela Lizeth Pérez-González,
Karla Juárez-Moreno,
Yanis Toledano-Magaña,
Elena Smolentseva,
Juan Antonio Paz-González,
Tatiana Popova,
Lyubov Rachkovskaya,
Vadim Nimaev,
Anastasia Kotlyarova,
Maksim Korolev,
Andrey Letyagin,
Luis Jesús Villarreal-Gómez
Affiliations
Alan Saúl Álvarez-Suárez
Facultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Valle de las Palmas, Mexico. Blvd. Universitario #1000, Unidad Valle de las Palmas, 22260 Tijuana, Baja California, Mexico
Syed G. Dastager
National Collection of Industrial Microorganisms (NCIM), CSIR-National Chemical Laboratory, Pune-411008, Maharashtra, India
Nina Bogdanchikova
Universidad Nacional Autónoma de México, Centro de Nanociencias y Nanotecnología, Km. 107, Carretera Tijuana a Ensenada, C.P. 22860 Ensenada, Baja California, Mexico
Daniel Grande
“Complex Polymer Systems” Laboratory, Institut de Chimie et des Matériaux Paris-Est, Université Paris-Est Créteil, UMR 7182 CNRS, 2, rue Henri Dunant, F-94320 Thiais, France
Alexey Pestryakov
Department of Technology of Organic Substances and Polymer Materials, Tomsk Polytechnic University, 634050 Tomsk, Russia
Juan Carlos García-Ramos
Escuela de Ciencias de la Salud, Universidad Autónoma de Baja California- Campus Valle Dorado, Carretera Transpeninsular S/N, Valle Dorado, 22890 Ensenada, Baja California, Mexico
Graciela Lizeth Pérez-González
Facultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Valle de las Palmas, Mexico. Blvd. Universitario #1000, Unidad Valle de las Palmas, 22260 Tijuana, Baja California, Mexico
Karla Juárez-Moreno
Universidad Nacional Autónoma de México, Centro de Nanociencias y Nanotecnología, Km. 107, Carretera Tijuana a Ensenada, C.P. 22860 Ensenada, Baja California, Mexico
Yanis Toledano-Magaña
Escuela de Ciencias de la Salud, Universidad Autónoma de Baja California- Campus Valle Dorado, Carretera Transpeninsular S/N, Valle Dorado, 22890 Ensenada, Baja California, Mexico
Elena Smolentseva
Universidad Nacional Autónoma de México, Centro de Nanociencias y Nanotecnología, Km. 107, Carretera Tijuana a Ensenada, C.P. 22860 Ensenada, Baja California, Mexico
Juan Antonio Paz-González
Facultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Valle de las Palmas, Mexico. Blvd. Universitario #1000, Unidad Valle de las Palmas, 22260 Tijuana, Baja California, Mexico
Tatiana Popova
Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630060 Novosibirsk, Russia
Lyubov Rachkovskaya
Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630060 Novosibirsk, Russia
Vadim Nimaev
Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630060 Novosibirsk, Russia
Anastasia Kotlyarova
Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630060 Novosibirsk, Russia
Maksim Korolev
Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630060 Novosibirsk, Russia
Andrey Letyagin
Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630060 Novosibirsk, Russia
Luis Jesús Villarreal-Gómez
Facultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Valle de las Palmas, Mexico. Blvd. Universitario #1000, Unidad Valle de las Palmas, 22260 Tijuana, Baja California, Mexico
Skin burns and ulcers are considered hard-to-heal wounds due to their high infection risk. For this reason, designing new options for wound dressings is a growing need. The objective of this work is to investigate the properties of poly (ε-caprolactone)/poly (vinyl-pyrrolidone) (PCL/PVP) microfibers produced via electrospinning along with sorbents loaded with Argovit™ silver nanoparticles (Ag-Si/Al2O3) as constituent components for composite wound dressings. The physicochemical properties of the fibers and sorbents were characterized using scanning electron microscopy (SEM), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR) and inductively coupled plasma optical emission spectroscopy (ICP-OES). The mechanical properties of the fibers were also evaluated. The results of this work showed that the tested fibrous scaffolds have melting temperatures suitable for wound dressings design (58–60 °C). In addition, they demonstrated to be stable even after seven days in physiological solution, showing no macroscopic damage due to PVP release at the microscopic scale. Pelletized sorbents with the higher particle size demonstrated to have the best water uptake capabilities. Both, fibers and sorbents showed antimicrobial activity against Gram-negative bacteria Pseudomona aeruginosa and Escherichia coli, Gram-positive Staphylococcus aureus and the fungus Candida albicans. The best physicochemical properties were obtained with a scaffold produced with a PCL/PVP ratio of 85:15, this polymeric scaffold demonstrated the most antimicrobial activity without affecting the cell viability of human fibroblast. Pelletized Ag/Si-Al2O3-3 sorbent possessed the best water uptake capability and the higher antimicrobial activity, over time between all the sorbents tested. The combination of PCL/PVP 85:15 microfibers with the chosen Ag/Si-Al2O3-3 sorbent will be used in the following work for creation of wound dressings possessing exudate retention, biocompatibility and antimicrobial activity.
