Stability and biological response of PEGylated gold nanoparticles
Hoang Yen Nguyenova,
Marie Hubalek Kalbacova,
Marcela Dendisova,
Miriama Sikorova,
Jaroslava Jarolimkova,
Zdenka Kolska,
Lucie Ulrychova,
Jan Weber,
Alena Reznickova
Affiliations
Hoang Yen Nguyenova
Department of Solid State Engineering, University of Chemistry and Technology Prague, 166 28, Prague, Czech Republic; Corresponding author.
Marie Hubalek Kalbacova
Institute of Pathological Physiology, 1st Faculty of Medicine, Charles University, 128 53, Prague, Czech Republic; Faculty of Health Studies, Technical University of Liberec, Liberec, Czech Republic
Marcela Dendisova
Department of Physical Chemistry, University of Chemistry and Technology Prague, 166 28, Prague, Czech Republic
Miriama Sikorova
Institute of Pathological Physiology, 1st Faculty of Medicine, Charles University, 128 53, Prague, Czech Republic
Jaroslava Jarolimkova
CENAB, Faculty of Science, J. E. Purkyne University, 400 96, Usti nad Labem, Czech Republic
Zdenka Kolska
CENAB, Faculty of Science, J. E. Purkyne University, 400 96, Usti nad Labem, Czech Republic
Lucie Ulrychova
Institute of Organic Chemistry and Biochemistry of the Czech Academy of Science, 166 10, Prague, Czech Republic
Jan Weber
Institute of Organic Chemistry and Biochemistry of the Czech Academy of Science, 166 10, Prague, Czech Republic
Alena Reznickova
Department of Solid State Engineering, University of Chemistry and Technology Prague, 166 28, Prague, Czech Republic; CENAB, Faculty of Science, J. E. Purkyne University, 400 96, Usti nad Labem, Czech Republic
Stability and cytotoxicity of PEGylated Au NPs is crucial for biomedical application. In this study, we have focused on thermal stability of PEGylated Au NPs at 4 and 37 °C and after sterilization in autoclave. Gold nanoparticles were prepared by direct sputtering of gold into PEG and PEG-NH2. Transmission electron microscopy revealed that NPs exhibit a spherical shape with average dimensions 3.8 nm for both AuNP_PEG and AuNP_PEG-NH2. The single LSPR band at wavelength of 509 nm also confirmed presence of spherical Au NPs in both cases. Moreover, according to UV–Vis spectra, the Au NPs were overall stable during aging or thermal stressing and even after sterilization in autoclave. Based on gel electrophoresis results, the higher density of functionalizing ligands and the higher stability is assumed on AuNP_PEG-NH2. Changes in concentration of gold did not occur after thermal stress or with aging. pH values have to be adjusted to be suitable for bioapplications – original pH values are either too alkaline (AuNP_PEG-NH2, pH 10) or too acidic (AuNP_PEG, pH 5). Cytotoxicity was tested on human osteoblasts and fibroblasts. Overall, both Au NPs have shown good cytocompatibility either freshly prepared or even after Au NPs′ sterilization in the autoclave. Prepared Au NP dispersions were also examined for their antiviral activity, however no significant effect was observed. We have synthesized highly stable, non-cytotoxic PEGylated Au NPs, which are ready for preclinical testing.
