MICROPROPAGAREA IN VITRO A SPARCETEI (Onobrychis viciifolia Scop.)

Abstract

IN VITRO MICROPROPAGATION OF SAINFOIN (ONOBRYCHIS VICIIFOLIA SCOP.) For the in vitro micropropagation there were used two types of explants, apexes and stem knots freshly inoculated. We took copse of different sizes, originating from plants situated in different years of vegetation (I, II, III). The incubated sainfoin knots and apexes were examined from the point of view of their in vitro behaviour regarding the regeneration, multiplication and striking root, depending on the hormonal balance from the culture medium and on the age of the donor plant. The viability and regeneration percentage of the sainfoin knot is comprised between 92 and 95 %, indifferently to the hormonal balance. The viability percentage of the apex is smaller than that of the knot, but its regeneration and multiplication capacity is higher. The regeneration degree is directly proportional with the age of the donor plant.