Comparison of Enzyme-Linked Immunosorbent Assay, Surface Plasmon Resonance and Biolayer Interferometry for Screening of Deoxynivalenol in Wheat and Wheat Dust
Melanie Sanders,
Daniel McPartlin,
Kara Moran,
Yirong Guo,
Mia Eeckhout,
Richard O’Kennedy,
Sarah De Saeger,
Chris Maragos
Affiliations
Melanie Sanders
Mycotoxin Prevention and Applied Microbiology Research Unit, United States Department of Agriculture-Agricultural Research Service-National Center for Agricultural Utilization Research (USDA-ARS-NCAUR), 1815 N. University Street, Peoria, IL 61604, USA
Daniel McPartlin
School of Biotechnology, National Centre for Sensor Research and Biomedical Diagnostic Institute, Dublin City University, Dublin 9, Ireland
Kara Moran
School of Biotechnology, National Centre for Sensor Research and Biomedical Diagnostic Institute, Dublin City University, Dublin 9, Ireland
Yirong Guo
Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China
Mia Eeckhout
Department of Applied Biosciences, Faculty of Bioscience Engineering, Ghent University, Valentin Vaerwyckweg 1, 9000 Ghent, Belgium
Richard O’Kennedy
School of Biotechnology, National Centre for Sensor Research and Biomedical Diagnostic Institute, Dublin City University, Dublin 9, Ireland
Sarah De Saeger
Department of Bioanalysis, Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium
Chris Maragos
Mycotoxin Prevention and Applied Microbiology Research Unit, United States Department of Agriculture-Agricultural Research Service-National Center for Agricultural Utilization Research (USDA-ARS-NCAUR), 1815 N. University Street, Peoria, IL 61604, USA
A sample preparation method was developed for the screening of deoxynivalenol (DON) in wheat and wheat dust. Extraction was carried out with water and was successful due to the polar character of DON. For detection, an enzyme-linked immunosorbent assay (ELISA) was compared to the sensor-based techniques of surface plasmon resonance (SPR) and biolayer interferometry (BLI) in terms of sensitivity, affinity and matrix effect. The matrix effects from wheat and wheat dust using SPR were too high to further use this screenings method. The preferred ELISA and BLI methods were validated according to the criteria established in Commission Regulation 519/2014/EC and Commission Decision 2002/657/EC. A small survey was executed on 16 wheat lots and their corresponding dust samples using the validated ELISA method. A linear correlation (r = 0.889) was found for the DON concentration in dust versus the DON concentration in wheat (LOD wheat: 233 μg/kg, LOD wheat dust: 458 μg/kg).
