Multiplex base editing to convert TAG into TAA codons in the human genome

Yuting Chen; Eriona Hysolli; Anlu Chen; Stephen Casper; Songlei Liu; Kevin Yang; Chenli Liu; George Church

doi:10.1038/s41467-022-31927-8

Nature Communications (Aug 2022)

Multiplex base editing to convert TAG into TAA codons in the human genome

Yuting Chen,
Eriona Hysolli,
Anlu Chen,
Stephen Casper,
Songlei Liu,
Kevin Yang,
Chenli Liu,
George Church

Affiliations

Yuting Chen: Department of Genetics, Harvard Medical School
Eriona Hysolli: Department of Genetics, Harvard Medical School
Anlu Chen: Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center, Harvard Medical School
Stephen Casper: Department of Genetics, Harvard Medical School
Songlei Liu: Department of Genetics, Harvard Medical School
Kevin Yang: Department of Genetics, Harvard Medical School
Chenli Liu: CAS Key Laboratory of Quantitative Engineering Biology, Center for Genome Engineering and Therapy, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
George Church: Department of Genetics, Harvard Medical School

DOI: https://doi.org/10.1038/s41467-022-31927-8
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

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Whole-genome recoding has been shown to enable nonstandard amino acids, biocontainment and viral resistance in bacteria. Here the authors extend this to human cells using base editing to convert TAG to TAA for 33 essential genes via a single transfection followed by examining base-editing genome-wide.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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