Zīst/shināsī-i Giyāhī-i Īrān (Mar 2015)
Effect of salt stress on protein pattern and some physiological parameters of Lycopersicon peruvianum under in vitro culture
Abstract
In this study, Lycopersicon peruvianum line 815 was grown on MS medium containing 0, 60, 90 and 120 mM NaCl. After 4 weeks of NaCl treatment dry and fresh weights, chlorophyll a, b, total chlorophyll and caretenoid, proline, sodium and potassium, , the total protein, SDS-PAGE pattern were studied. The results showed that dry and fresh weight in shoot and root decreased with increasing of NaCl concentration. Chlorophyll and carotenoid pigments did not show any significant difference between the control and the treated explants. Proline and sodium contents increased with salinity compared to the control, but the potassium level and total protein content decreased. K+/Na+ ratio decreased with increasing of salt stress. In addition, SDS-PAGE results showed different protein pattern between the treated and the untreated plants. According to the mentioned results salt stress could directly affect plant growth and it had indirect on metabolism of plant.
