Improving immunoassay detection accuracy of anti-SARS-CoV-2 antibodies through dual modality validation

Yuhao Ma; Daniel To; Jie Zeng; Lian C.T. Shoute; Meng Wu; Shawn Babiuk; Ran Zhuo; Carmen Charlton; Jamil N. Kanji; Lorne Babiuk; Jie Chen

Biosensors and Bioelectronics: X (Sep 2022)

Improving immunoassay detection accuracy of anti-SARS-CoV-2 antibodies through dual modality validation

Yuhao Ma,
Daniel To,
Jie Zeng,
Lian C.T. Shoute,
Meng Wu,
Shawn Babiuk,
Ran Zhuo,
Carmen Charlton,
Jamil N. Kanji,
Lorne Babiuk,
Jie Chen

Affiliations

Yuhao Ma: Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB, T6G 2V4, Canada
Daniel To: Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB, T6G 2V4, Canada
Jie Zeng: Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB, T6G 2V4, Canada
Lian C.T. Shoute: Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB, T6G 2V4, Canada
Meng Wu: Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, T6G 2R3, Canada
Shawn Babiuk: National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada; Department of Immunology, University of Manitoba, Winnipeg, MB, Canada
Ran Zhuo: Public Health Laboratory, Alberta Precision Laboratories, Calgary, AB, Canada
Carmen Charlton: Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, T6G 2B7, Canada; Public Health Laboratory, Alberta Precision Laboratories, Calgary, AB, Canada; Li Ka Shing Institute for Virology, University of Alberta, Edmonton, AB, Canada
Jamil N. Kanji: Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, T6G 2B7, Canada; Public Health Laboratory, Alberta Precision Laboratories, Calgary, AB, Canada; Division of Infectious Diseases, Department of Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada; Department of Pathology & Laboratory Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada
Lorne Babiuk: Vaccine and Infectious Disease Organization, University of Alberta, Edmonton, AB, Canada
Jie Chen: Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB, T6G 2V4, Canada; Department of Biomedical Engineering, University of Alberta, Edmonton, AB T6G 2V2, Canada; Corresponding author. Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB, T6G 2V4, Canada.

Journal volume & issue: Vol. 11
p. 100176

Abstract

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A novel test strategy is proposed with dual-modality detection techniques for COVID-19 antibody detection. The full-length S protein of SARS-CoV-2 was chemically immobilized on a glass surface to capture anti-SARS-CoV-2 IgG in patient serum and was detected through either Electrochemical Impedance Spectroscopy (EIS) or fluorescence imaging with labeled secondary antibodies. Gold nanoparticles conjugated with protein G were used as the probe and the bound GNP-G was detected through EIS measurements. Anti-human-IgG conjugated with the fluorescent tag Alexa Fluor 488 was used as the probe for fluorescence imaging. Clinical SARS-CoV-2 IgG positive serum and negative controls were used to validate both modalities. For fluorescence-based detection, a high sensitivity was noticed with a quantification range of 0.01–0.1 A.U.C. and a LOD of 0.004 A.U.C. This study demonstrates the possibility of utilizing different measurement techniques in conjunction for improved COVID-19 serology testing.

Published in Biosensors and Bioelectronics: X

ISSN: 2590-1370 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://www.journals.elsevier.com/biosensors-and-bioelectronics-x

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