Molecular Detection of Toxoplasma gondii among Children Attending Selected Schools in Kaduna State, Nigeria

Lawal, S.M.; Shitu, S.; Hussaini, I.M.

doi:10.47430/ujmr.1832.013

UMYU Journal of Microbiology Research (Dec 2018)

Molecular Detection of Toxoplasma gondii among Children Attending Selected Schools in Kaduna State, Nigeria

Lawal, S.M.,
Shitu, S.,
Hussaini, I.M.

Affiliations

Lawal, S.M.: Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria
Shitu, S.: Scientific and Industrial Research Department, National Research Institute for Chemical Technology, Zaria.
Hussaini, I.M.: Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria

DOI: https://doi.org/10.47430/ujmr.1832.013
Journal volume & issue: Vol. 3, no. 2

Abstract

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Toxoplasmosis is a zoonotic infection of both humans and animals and has a worldwide distribution. A total of 300 blood samples were collected from school children in Kaduna state. The sera were screened for Toxoplasma gondii IgM and IgG antibodies by Enzyme Linked Immunosorbent Assay (ELISA). The seropositive samples were screened for T. gondii specific B1 gene. Out of the 300 blood samples screened for Toxoplasma IgM antibody, 108 (36.0%) were positive while 50 (16.67%) were seropositive for the Toxoplasma IgG antibody. So also 24 (8.0%) samples were seropositive for ToxoplasmaIgG+IgM antibodies. B1 gene specific forT. gondiiwas detected by PCRin the ELISA positive samples.This confirms the presence of the parasite among the school children in Kaduna state.

Published in UMYU Journal of Microbiology Research

ISSN: 2616-0668 (Print); 2814-1822 (Online)
Publisher: Umaru Musa Yar'adua University, Katsina, Nigeria
Country of publisher: Nigeria
LCC subjects: Science: Microbiology
Website: https://ujmr.umyu.edu.ng/index.php/ujmr/

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