A three-layered hydrogel patch with hierarchy releasing of PLGA nanoparticle drugs decrease neointimal hyperplasia

Shunbo Wei; Jing’an Li; Hao He; Chang Shu; Alan Dardik; Hualong Bai

Smart Materials in Medicine (Jan 2022)

A three-layered hydrogel patch with hierarchy releasing of PLGA nanoparticle drugs decrease neointimal hyperplasia

Shunbo Wei,
Jing’an Li,
Hao He,
Chang Shu,
Alan Dardik,
Hualong Bai

Affiliations

Shunbo Wei: Department of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Henan, China; Key Vascular Physiology and Applied Research Laboratory of Zhengzhou City, Henan, China
Jing’an Li: School of Material Science and Engineering & Henan Key Laboratory of Advanced Magnesium Alloy & Key Laboratory of Materials Processing and Mold Technology (Ministry of Education), Zhengzhou University, Henan, China
Hao He: Department of Vascular Surgery, the Second Xiangya Hospital, Central South University, Changsha, China
Chang Shu: Department of Vascular Surgery, the Second Xiangya Hospital, Central South University, Changsha, China; Department of Vascular Surgery, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
Alan Dardik: The Vascular Biology and Therapeutics Program, Yale School of Medicine, New Haven, CT, USA; Departments of Surgery and of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, CT, USA
Hualong Bai: Department of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Henan, China; Key Vascular Physiology and Applied Research Laboratory of Zhengzhou City, Henan, China; Corresponding author. Department of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Longhuzhonghuan Road, Zhengdongxinqu, Zhengzhou, Henan, 450001, China.

Journal volume & issue: Vol. 3
pp. 139 – 147

Abstract

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Hydrogel is a nature scaffold that can degraded in animal body and can be used as a drug delivery system, we hypothesized that patch made of three layers of hydrogel with different PLGA nanoparticle drugs (bio-patch) can be used to decrease venous neointimal hyperplasia. Rat inferior vena cava (IVC) patch venoplasty model was used. Samples from rat IVC direct suture (DS), decellularized thoracic artery patch (TA) venoplasty and bovine pericardial patch (BPP) venoplasty were examined at day 14 after implantation. Sodium alginate and hyaluronic acid (SA/HA) hydrogel was used, three layers hydrogel patch (control) and three layers hydrogel patch with PLGA nanoparticle drugs (bio-patch) were used in rat IVC venoplasty. Patches were harvested at day 14 and analyzed. In rats, TA and BPP patch showed a thicker neointima and adventitia compared to the DS, there were larger numbers of CD68 and PCNA positive cells in both groups. The control hydrogel patch showed much thinner neointima and adventitia compared to TA and BPP patches. In both of the neointima and peri-patch area, bio-patch showed significantly fewer smooth muscle cells, fewer CD68, fewer PCNA positive cells, fewer collagen-1 positive cells, fewer p-smad2 positive cells, fewer TNF-α positive cells compared to control hydrogel patch. Bio-patch made of hydrogel and PLGA nanoparticle drugs showed a thinner neointimal thickness, and is biocompatible to the animal body. These results showed the potential application of hydrogel patch in vascular surgery.

Published in Smart Materials in Medicine

ISSN: 2590-1834 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology
Website: https://www.keaipublishing.com/en/journals/smart-materials-in-medicine/

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