PLoS Biology (Aug 2019)

Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice.

  • Lech Kaczmarczyk,
  • Vikas Bansal,
  • Ashish Rajput,
  • Raza-Ur Rahman,
  • Wiesław Krzyżak,
  • Joachim Degen,
  • Stefanie Poll,
  • Martin Fuhrmann,
  • Stefan Bonn,
  • Walker Scot Jackson

DOI
https://doi.org/10.1371/journal.pbio.3000374
Journal volume & issue
Vol. 17, no. 8
p. e3000374

Abstract

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A deep understanding of how regulation of the multiple levels of gene expression in mammalian tissues give rise to complex phenotypes has been impeded by cellular diversity. A handful of techniques were developed to tag-select nucleic acids of interest in specific cell types, thereby enabling their capture. We expanded this strategy by developing the Tagger knock-in mouse line bearing a quad-cistronic transgene combining enrichment tools for nuclei, nascent RNA, translating mRNA, and mature microRNA (miRNA). We demonstrate that Tagger can capture the desired nucleic acids, enabling multiple omics approaches to be applied to specific cell types in vivo using a single transgenic mouse line.