A Simple Entropic‐Driving Separation Procedure of Low‐Size Silver Clusters, Through Interaction with DNA
Dr. David Buceta,
Dr. Blanca Dominguez,
Dr. Sara Vieitez,
Dr. Iria R. Arias,
Dr. J. Manuel Ageitos,
Prof. M. Carmen Blanco,
Prof. Giampaolo Barone,
Prof. Fernando Domínguez,
Prof. M. Arturo López‐Quintela
Affiliations
Dr. David Buceta
Department of Physical Chemistry Lab. Nanomag University of Santiago de Compostela 15782 Santiago de Compostela Spain
Dr. Blanca Dominguez
Department of Physical Chemistry Lab. Nanomag University of Santiago de Compostela 15782 Santiago de Compostela Spain
Dr. Sara Vieitez
Department of Physical Chemistry Lab. Nanomag University of Santiago de Compostela 15782 Santiago de Compostela Spain
Dr. Iria R. Arias
Department of Physical Chemistry Lab. Nanomag University of Santiago de Compostela 15782 Santiago de Compostela Spain
Dr. J. Manuel Ageitos
Department of Pharmacology Pharmacy and Pharmaceutical Technology and Centro de Investigaciones en Medicina Molecular y Enfermedades Crónicas (CIMUS) University of Santiago de Compostela 15782 Santiago de Compostela Spain
Prof. M. Carmen Blanco
Department of Physical Chemistry Lab. Nanomag University of Santiago de Compostela 15782 Santiago de Compostela Spain
Prof. Giampaolo Barone
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies University of Palermo 90128 Palermo Italy
Prof. Fernando Domínguez
Department of Physiology and Centro de Investigaciones en Medicina Molecular y Enfermedades Crónicas (CIMUS) University of Santiago de Compostela E-15782 Santiago de Compostela Spain
Prof. M. Arturo López‐Quintela
Department of Physical Chemistry Lab. Nanomag University of Santiago de Compostela 15782 Santiago de Compostela Spain
Abstract Synthesis and purification of metal clusters without strong binding agents by wet chemical methods are very attractive for their potential applications in many research areas. However, especially challenging is the separation of uncharged clusters with only a few number of atoms, which renders the usual techniques very difficult to apply. Herein, we report the first efficient separation of Ag2 and Ag3 clusters using the different entropic driving forces when such clusters interact with DNA, into which Ag3 selectively intercalates. After sequential dialysis of the samples and denaturalizing the DNA‐Ag3 complex, pure Ag2 can be found in the dialysate after extensive dialysis. Free Ag3 is recovered after DNA denaturation.
