Comprehensive Analysis of Autophagy-Related Genes in Rice Immunity against <i>Magnaporthe oryzae</i>
Xuze Xie,
Mengtian Pei,
Shan Liu,
Xinxiao Wang,
Shanshan Gong,
Jing Chen,
Ye Zhang,
Zonghua Wang,
Guodong Lu,
Ya Li
Affiliations
Xuze Xie
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Mengtian Pei
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Shan Liu
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Xinxiao Wang
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Shanshan Gong
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Jing Chen
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Ye Zhang
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Zonghua Wang
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Guodong Lu
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Ya Li
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Rice blast disease, caused by the fungus Magnaporthe oryzae, is a significant threat to rice production. Resistant cultivars can effectively resist the invasion of M. oryzae. Thus, the identification of disease-resistant genes is of utmost importance for improving rice production. Autophagy, a cellular process that recycles damaged components, plays a vital role in plant growth, development, senescence, stress response, and immunity. To understand the involvement of autophagy-related genes (ATGs) in rice immune response against M. oryzae, we conducted a comprehensive analysis of 37 OsATGs, including bioinformatic analysis, transcriptome analysis, disease resistance analysis, and protein interaction analysis. Bioinformatic analysis revealed that the promoter regions of 33 OsATGs contained cis-acting elements responsive to salicylic acid (SA) or jasmonic acid (JA), two key hormones involved in plant defense responses. Transcriptome data showed that 21 OsATGs were upregulated during M. oryzae infection. Loss-of-function experiments demonstrated that OsATG6c, OsATG8a, OsATG9b, and OsATG13a contribute to rice blast resistance. Additionally, through protein interaction analysis, we identified five proteins that may interact with OsATG13a and potentially contribute to plant immunity. Our study highlights the important role of autophagy in rice immunity and suggests that OsATGs may enhance resistance to rice blast fungus through the involvement of SA, JA, or immune-related proteins. These findings provide valuable insights for future efforts in improving rice production through the identification and utilization of autophagy-related genes.