Cancer Management and Research (Feb 2020)
Effects of Topical Hangeshashinto (TJ-14) on Chemotherapy-Induced Oral Mucositis
Abstract
Natsuo Ozawa,1 Takeshi Onda,1 Kamichika Hayashi,1 Hirona Honda,1 Takahiko Shibahara1,2 1Department of Oral and Maxillofacial Surgery, Tokyo Dental College, Chiba 261-8502, Japan; 2Oral Cancer Center, Tokyo Dental College, Chiba 272-8513, JapanCorrespondence: Takeshi OndaDepartment of Oral and Maxillofacial Surgery, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-8502, JapanTel +81-43-270-3978Fax +81-43-270-3979Email [email protected]: Hangeshashinto (TJ-14), a Kampo medicine comprising seven types of herbs, has been used in Japan to alleviate the side effects associated with anticancer drug treatments. However, the pharmacological effects of this medicine currently remain unclear. The present study aimed to demonstrate the efficacy of TJ-14 against anticancer drug-induced stomatitis, the pain associated with which may have a negative impact on mastication and swallowing.Methods: Mucositis was induced in Sprague-Dawley rats by cancer chemotherapy. Changes in body weight, stomatitis grades, histopathological scores, and oral bacterial counts were examined among TJ-14-treated, saline-treated, and Control (no treatment) rats. In vitro studies, including cell proliferation and wound healing assays, using epidermal keratinocyte and fibroblast cell lines were conducted.Results: The local application of TJ-14 exerted strong antibacterial effects and attenuated oral chemotherapy-induced stomatitis in rats. TJ-14 also increased the viability and invasion of epidermal keratinocytes and fibroblasts.Conclusion: The present results demonstrated the potential of TJ-14 to attenuate chemotherapy-induced stomatitis.Keywords: cancer chemotherapy, mucositis, Hangeshashinto, TJ-14, Kampo medicine, traditional Japanese medicine, oral cancer
