Development of an Antioxidant, Anti-Aging, and Photoprotective Phytocosmetic from Discarded <i>Agave sisalana</i> Perrine Roots
Guilherme dos Santos Mazo,
Julia Amanda Rodrigues Fracasso,
Luísa Taynara Silvério da Costa,
Valdecir Farias Ximenes,
Natália Alves Zoppe,
Amanda Martins Viel,
Lucas Pires Guarnier,
Beatriz de Castro Silva,
Luan Victor Coelho de Almeida,
Lucinéia dos Santos
Affiliations
Guilherme dos Santos Mazo
Department of Biotechnology, School of Sciences and Languages, São Paulo State University (UNESP), 2100, Dom Antonio Avenue, Assis 19806-900, SP, Brazil
Julia Amanda Rodrigues Fracasso
School of Dentistry, São Paulo State University (UNESP), 1193, José Bonifacio Street, Araçatuba 16015-050, Brazil
Luísa Taynara Silvério da Costa
School of Dentistry, São Paulo State University (UNESP), 1193, José Bonifacio Street, Araçatuba 16015-050, Brazil
Valdecir Farias Ximenes
Department of Chemistry, Faculty of Sciences, São Paulo State University (UNESP), Bauru 17033-360, SP, Brazil
Natália Alves Zoppe
Department of Biotechnology, School of Sciences and Languages, São Paulo State University (UNESP), 2100, Dom Antonio Avenue, Assis 19806-900, SP, Brazil
Amanda Martins Viel
São Camilo University Center, Sao Paolo 04263-200, SP, Brazil
Lucas Pires Guarnier
Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14048-900, SP, Brazil
Beatriz de Castro Silva
Department of Biotechnology, School of Sciences and Languages, São Paulo State University (UNESP), 2100, Dom Antonio Avenue, Assis 19806-900, SP, Brazil
Luan Victor Coelho de Almeida
Department of Biotechnology, School of Sciences and Languages, São Paulo State University (UNESP), 2100, Dom Antonio Avenue, Assis 19806-900, SP, Brazil
Lucinéia dos Santos
Department of Biotechnology, School of Sciences and Languages, São Paulo State University (UNESP), 2100, Dom Antonio Avenue, Assis 19806-900, SP, Brazil
The primary source of hard fiber globally is Agave sisalana Perrine, also known as sisal. In areas where sisal is grown, the roots of the plant are usually left in the field after it has stopped producing, which leads to soil degradation and decreased sisal productivity. It is, therefore, critical to find alternatives to reuse this waste. This study explores the potential use of sisal waste in the cosmetic industry by incorporating a hydroethanolic extract (HER) into a cream–gel formulation, taking advantage of the plant’s recognized ethnopharmacological value. The study involves analyzing the extract’s phytochemical composition (flavonoids) and evaluating its cytotoxicity. Subsequently, the antioxidant and antiglycation activities of the extract and cream–gel are evaluated, as well as ex vivo ocular toxicity, photoprotective activity, and preliminary stability analyses. The HER extract showed a flavonoid composition (catechin, kaempferol, isorhamnetin, and chrysin) and maintained cell viability above 70% throughout all time points analyzed in the MTT assay. Furthermore, the extract and the formulation demonstrated proven antioxidant and antiglycation activities. The cream–gel’s UVB and UVA protection effectiveness with the HER was comparable to that of synthetic UVB/UVA sunscreens, with the samples proving nonirritating and stable. In conclusion, the extract has a significant presence of flavonoids, and the cream–gel developed with it did not present cytotoxicity and met the stability requirements, indicating phytocosmetic potential with antioxidant, antiglycation, and photoprotective properties.
