International Journal of General Medicine (May 2021)

Transperineal Parallel Biopsy of the Prostate: A New Approach of Tissue Sampling for Precision Medicine

  • Qu M,
  • Lian B,
  • Wang Y,
  • Zhang W,
  • Zhu F,
  • Wang T,
  • Yue X,
  • Jia Z,
  • Chen H,
  • Li H,
  • Li J,
  • Gao X

Journal volume & issue
Vol. Volume 14
pp. 1631 – 1640

Abstract

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Min Qu,1,* Bijun Lian,1,2,* Yan Wang,1,* Wenhui Zhang,1 Feng Zhu,1,3 Tao Wang,4 Xiaodong Yue,5 Zepeng Jia,1 Huan Chen,1 Husheng Li,1 Jing Li,1,6 Xu Gao1 1Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai, People’s Republic of China; 2Department of Urology, The 903th PLA Hospital, Hangzhou, People’s Republic of China; 3Department of Urology, Tianyou Hospital, Tongji University, Shanghai, People’s Republic of China; 4Department of Urology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, People’s Republic of China; 5Shanghai Institute for Advanced Communication and Data Science, Shanghai University, Shanghai, People’s Republic of China; 6Center for Translational Medicine, Second Military Medical University, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jing LiCenter for Translational Medicine, Second Military Medical University, No. 800 Xiangyin Road, Yangpu District, Shanghai, 200438, People’s Republic of ChinaTel +86 021-31161718Email [email protected] GaoDepartment of Urology, Changhai Hospital, No. 168 Changhai Road, Yangpu District, Shanghai, 200438, People’s Republic of ChinaTel +86 021-31161717Email [email protected]: Through an observational study to present a new approach for obtaining high-quality samples for the targeted therapy of prostate cancer.Patients and Methods: Parallel biopsy, which was defined as collecting the tissue from the same site by two biopsies, was performed on patients with elevated PSA. Each tissue was stained by ink to identify the pathological characteristics, including Gleason score and tumor tissue ratio. Kendall tau-b test and intraclass correlation coefficient test were used to compare the consistency between each paired sample. Then, based on the pathology of the biopsies, high-quality tissues would be selected for sequencing, and PyClone model was used to track the clonal evolution.Results: In total, 252 pairs of biopsies were collected. The consistency of Gleason score between each paired biopsy is 0.777 (p< 0.01), and the consistency of tumor tissue ratio is 0.853 (p< 0.01). With the application of parallel biopsy, on average five nonsynonymous mutations could be identified in patients with castration-resistant prostate cancer. Six out of eight had at least one biology-relevant alteration in patients, guiding further treatment. Meanwhile, clonal evolution was constructed to investigate the progress of tumor.Conclusion: Parallel biopsy is a reliable approach to collect high-quality tissue and shows potential application in precision medicine.Keywords: prostate cancer, prostate biopsy, next-generation sequencing, clonal evolutionary tree, precision medicine

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