Gelatinolytic activity in dentin upon adhesive treatment

Abstract

Abstract In this multi-parameter study, the effect of diverse factors related to adhesive application on the activation of host-derived gelatinases was investigated by gelatin zymography, in-situ zymography, fluorogenic DQ-gelatin assay and micro-tensile bond-strength (μTBS) testing. Gelatin zymography disclosed the presence of gelatinases in phosphoric acid-etched dentin powder, while two gold-standard adhesives generated no measurable MMP activation. In-situ zymography revealed that the interfacial gelatinolytic activity from specimens treated with the two adhesives appeared similar as that of the EDTA negative control, indicating no detectable gelatinases were activated upon adhesive treatment. In solution, MMP-2/9 activity significantly decreased upon interaction with both adhesives (two-way linear mixed effects model [LMEM]: p 0.05). Taken together, the two adhesives did not activate but rather inhibited the release and activation of dentinal gelatinases.

Keywords

• Dentin-bonding agents
• Matrix metalloproteinase 2
• Matrix metalloproteinase 9
• Gelatinases
• Dental bonding
• Biodegradation